COMPARISON RESULTS OF ABO BLOOD CLASS EXAMINATION AGLUTINATION WITH ANTISERA REAGENTS BEFORE EXPIRED AND ANTISERA REAGENTS EXPIRED IN THE LABORATORY OF REGIONAL HEALTH, GORONTALO PROVINCE

  • Fauzia Hasan Universitas Bina Mandiri Gorontalo
Keywords: agglutination, unexpired-expired reagent, blood group

Abstract

Examination of ABO blood groups in general uses the slide method, in the examination of blood groups using antisera A and antisera reagents. Antisera reagents for blood group tests that have passed the expiration date can give less accurate results for an examination, while antisera reagents before expiration date also can provide agglutination results in accordance with a person's blood type. This type of research is quantitative analytic research with a cross sectional research design.

The sampling technique was accidental sampling with a sample size of 19 respondents from the Unexpired antisera reagent and expired antisera reagent, then compared using the Mann Whitney test.

The results showed that there was no significant difference (0.000 <0.05) between unexpired and expired antisera reagents, so it was continued in the calculation of the U value to determine the decisions of H0 and H1, the value of U1 and the value of U2 were taken the smallest values ​​to be compared with U. table. Obtained U value table is 123. Because U count(48) <(123) then the H0 hypothesis is rejected and the H1 hypothesis is accepted with the conclusion that the results of the agglutination levels of blood group examination using unexpired antisera reagents are different from using expired antisera reagents.

Published
2023-10-17