TEST OF THE EFFECTIVENESS OF ALOE VERA LEAF EXTRACT (Aloevera) IN THE HEALING OF CUT WOUNDS IN MALE WHITE RATS (Rattus Norvegicus)

Abstract

Aloe vera is a medicinal plant widely recognized for its antioxidant, anti-inflammatory, and antimicrobial properties, making it a promising natural agent for wound healing. This study aimed to evaluate the effectiveness of Aloe vera leaf extract in promoting the healing of incision wounds in male white rats (Rattus norvegicus). A true experimental study with a post-test only control group design was conducted using 15 male white rats divided into five groups: positive control, negative control, and three treatment groups receiving different concentrations of Aloe vera leaf extract. The extract was prepared by ethanol maceration and subjected to qualitative phytochemical screening. Wound healing was evaluated based on wound diameter, pus resolution, swelling reduction, and healing time. Data were analyzed using One-Way ANOVA and the Kruskal–Wallis test at a significance level of p < 0.05. The extraction process produced a 14% yield, and phytochemical screening confirmed the presence of flavonoids, saponins, tannins, and polyphenols. Aloe vera leaf extract had no significant effect on wound diameter (p = 0.391). However, it significantly accelerated pus resolution (p = 0.011), swelling reduction (p = 0.009), and overall healing time (p = 0.024). Higher extract concentrations produced more favorable healing outcomes than lower concentrations. Aloe vera leaf extract effectively promoted incision wound healing by reducing inflammation-related parameters and shortening healing time, indicating its potential as a natural therapeutic agent for wound management.