ACTIVITY TEST OF LEMONGRASS LEAF EXTRACT (CYMBOPOGON CITRATUS) AGAINST STREPTOCOCCUS MUTANS BACTERIA AND ESCHERCHIA COLI

Nurul  Sakinah

Nurul Sakinah Bina Mandiri Gorontalo University

Keywords: Lemongrass Leaf Extract (Cymbopogon citratus), Antibacterial Activity, Streptococcus mutans bacteria. and Escherchia coli

Abstract

ABSTRACT

This study aims to determine the antibacterial activity of lemongrass leaf extract (Cymbopogon citratus) against Streptococcus mutans and Escherchia coli bacteria. This research method uses a Complete Randomized Design (RAL) consisting of 7 treatments and 4 repeats. The treatments are: P1 (negative control), P2 (10% concentration), P3 (20% concentration), P4 (30% concentration), P5 (40% concentration), P6 (50% concentration) and P7 (Amoxicillin positive control). The results showed that the concentration of lemongrass leaf extract (Cymbopogon citratus) has activity to inhibit the growth of Streptococcus mutans and Escherchia coli bacteria. The results of the statistical analysis of the Kruskall Wallis test showed a significant value of 0.008 or <0.05, so it can be said that there is a dose of lemongrass leaves (Cymbopogon citratus) against the growth of Streptococcus mutans bacteria. The processed data using the Kruskall Wallis test on Escherchia coli bacteria showed a significant value of 0.002 or <0.05, so it can be said that there is a dose of lemongrass leaves (Cymbopogon citratus) against Escherchia coli bacteria. Further tests (Duncan Test) found that there was no significant difference between one concentration and another, so it can be said that the optimum concentration of lemongrass leaf extract treatment (Cymbopogon citratus) in inhibiting the growth of Streptococcus mutans and Escherchia coli bacteria is at a concentration of 50%

References

1. Lemongrass leaf extract (Cymbopogon citratus) has antibacterial activity against Streptococcus mutans and Escherchia coli bacteria
2. The optimum concentration of lemongrass leaf extract (Cymbopogon citratus) which has antibacterial activity against Streptococcus mutans and Escherchia coli bacteria is at a concentration of 50%

REFERENCE
[1] Adamczak, Artur & Ożarowski, Marcin & Tomasz M. Karpiński. 2019. Antibacterial Activity of Some Flavonoids and Organic Acids Widely Distributed in Plants. Journal of Clinical Medicine. Vol 9 (1)
[2] Afifah Rukmini. (2020). Skrining Fitokimia Familia Piperaceae. Jurnal Biologi Dan Pembelajarannya (JB&P), 7(1), 28–32..
[3] Amaliah ZZN, Bahri S, dan Amelia P, 2018. Isolasi dan Karakterisasi Bakteri Asam Laktat dari Limbah Cair Rendaman Kacang Kedelai. Jurnal Agroekoteknologi; 5(1): 253–257
[4] Aulia, R. R., Astannudiansyah, dan Abdul, B. 2019. Faktor-faktor Yang Berhubungan Denga Status Karies Gigi Pada Anak Sekolahan Man 1 Kota Banjarmasin. Jurnal Kesehatan Indonesia. Volume IX Nomor 3. STIKES Cahaya Bangsa Banjarmasin: Banjar
[5] Azizah, Z., Misfadhila, S., dan Oktoviani, T. S. 2019. "Skrining Fitokimia dan UjiAktivitas Antioksidan Ekstrak Metanol Bubuk Kopi Olahan TradisionalSungai Penuh-Kerinci Dan Teh Kayu Aro Menggunakan Metode DPPH ( 1, 1-Difenil-2-Pikrilhidrazil )". Jurnal Farmasi Higea. Vol. 11 (2) :105-112
[6] Balfas, Rifqi Ferry & Rahmawati ,Yuniarti Dewi .2022. Skrining Fitokimia, Formulasi, dan Uji Sifat Fisik Sediaan Foot Sanitizer Spray Minyak Atsiri Sereh Wangi (Cymbopogon citratrus sp.) Jurnal Pharmascience, Vol. 9, No(2)
[7] Botahala L. (2021). Pembuatan HerbalSiap Saji Di Masa Pandemi CoViD-19. Abdimas Unwahas. Vol 6 (1)
[8] Elsya, N. M., Fitrianti, D., & Gita, C. E. D. 2020. Skrining Fitokimia Senyawa Metabolit Sekunder dari Simplisia dan Ekstrak Air Daun Bidara Arab (Ziziphus spina-christi L.). Prosiding Farmasi. Vol 6 (1)
[9] Fadilah. 2018. Uji Aktivitas Antibakteri Ekstrak Daun Kelor (Moringa oleifera L.) Terhadap Penyembuhan Luka Pada Mencit (Mus muscullus L.). Skripsi Universitas Sumatera Utara : Medan
[10] Febria, Whika & Rumiyanti, Leni & Rakhmawati, Ismi. 2020. Rendemen dan Skrining Fitokimia pada Ekstrak Daun Sanseviera sp. Jurnal Penelitian Pertanian Terapan Vol 17 (3)
[11] Fitriana, Y.A.N., Fatimah, V.A.N., Dan Fitri, A.S. 2019. Aktivitas Antibakteri Daun Sirih : Uji Ekstrak KHM (Kadar Hambat Minimum) Dan KBM (Kadar Bakterisidal Minimum),Vol 16 (2)
[12] Jayanti, Devi Dwi & Susanti, R. & Yuniastuti, Ari & Suardana, I Wayan. 2020. Deteksi Escherichia coli O157 pada Air Minum di Kelurahan Sekaran Gunungpati Semarang. Jurnal Biologi Udayana. Vol 24 (2).
[13] Joegijantoro, Rudy. 2019. Penyakit Infeksi. Malang: Intimedia
[14] Julianto, Tatang Shabur. 2019. Fitokimia Tinjauan Metabolit Sekunder dan Skrining Fitokimia. Jakarta
[15] Kemenkes RI. 2020. Profil Kesehatan Indonesia Tahun 2019. Kementrian Kesehatan Republik Indonesia. Jakarta.
[16] Kirtanayasa, Ayuning. 2022. Aktivitas Antibakteri Beberapa Ekstrak Tanaman Terhadap Bakteri Klebsiella Pneumonia. Jurnal Gema Agro. Vol 27 (2)
[17] Lidyawati & Nurul Hidayati, Ria Ceriana. 2021. Formulasi Sediaan Salep Dari Ekstrak Daun Katuk (Sauropus androgynus L.) merr.) Journal of Pharmaceutical and Health Research. Vol 2 (3)
[18] Manongkoa, Paricia Syaron & Sangia, Meiske Sientje & Momuata, Lidya Irma. 2020. Uji Senyawa Fitokimia dan Aktivitas Antioksidan Tanaman Patah Tulang (Euphorbia tirucalli L.), Jurnal Mipa. Vol 9 (2)
[19] Prasetya, Yulianto Ade & Winarsih, Ike Yuyun & Pratiwi, Kharisma Aprilia. 2019. Deteksi Fenotipik Escherichia coli Penghasil Extended Spectrum Beta-Lactamases (Esbls) Pada Sampel Makanan Di Krian Sidoarjo. Jurnal Life Science. Vol 8 (1)
[20] Pujawati, R.S., Rahmat, M., Djuminar, A., & Rahayu, I.G. 2019. Uji Efektivitas Ekstrak Serai Dapur (Cymbopogon Citratus (Dc.) Stapf) Terhadap Pertumbuhan Candida Albicans Metode Makrodilusi. Jurnal Riset Kesehatan Poltekkes Kemenkes Bandung. Vol. 11, No. 2.
[21] Rini, C. S., & Rochmah, J. (2020). Bakteriologi Dasar. Sidoarjo: UMSIDA Press
[22] Safrudin, N. & F. Nurfitasari. 2018. Analisis senyawa metabolit sekunder dan uji aktivitas antioksidan dengan metode DPPH (1,1-diphenyl-2- picrylhydrazyl) dari ekstrak daun bidara (Ziziphus spina-christi L.). Jurnal Itekima. Vol 4 (2)
[23] Saranraj, P., & Devi, D. (2018). Essential Oils and its Antibacterial Properties-A Review. Life Science Archives ( LSA ) REVIEW, 3, 848–853.
[24] Sudiarto & Saleh, Rsmiati & Sawab. 2022. Kandungan Nutrisi Minuman Herbal Fungsinal Berbahan Dasar Gula Semut Aren dan Serbuk Rempah. Jurnal Teknologi Pertanian. Vol 11 (2)
[25] Winato B.M, Sanjaya, Siregar, Fau, Mutia. 2019. Uji Aktivitas Antibakteri Ekstrak Daun Serai Wangi (Cymbopogon nardus L.) terhadap Bakteri Propionabacterium Acnes.Jurnal Biologi Lingkungan, Industri dan Kesehatan, Vol. (1) Agustus 2019.
[26] Yudhi Nuryadin, Tadjuddin Naid, Andi Amaliah Dahlia, Seniwati Dali. 2019. Kadar Flavonoid Total Ekstrak Etanol Daun Serai Dapur dan Daun Alang-Alang Menggunakan Spektrofotometri UV-VIS. Jurnal Kesehatan, Vol. 1 No. 4 (Oktober, 2018)