CENTRIFUGE TIME VARIATIONS ON RECALCIFICATION PERIOD VALUE

Fathan Mantu; Rita Amini  Warastuti; Marlia

doi:10.47918/jhts.v5i2.1784

Fathan Mantu Universitas Bina Mandiri Gorontalo, indonesia
Rita Amini Warastuti Universitas Bina Mandiri Gorontalo, Indonesia
Marlia Faisal Islamic hospital

DOI: https://doi.org/10.47918/jhts.v5i2.1784

Keywords: Centrifuge Time, Recalcification Period

Abstract

Hemostasis is the body’s mechanism to stop bleeding during an injury. The recalcification period examination is a specific hematological examination of blood clotting disorders to look for deficiency of clotting factors from the intrinsic pathway (Factors V, VIII, IX, X, XI, and XII), prothrombin and fibrinogen. According to the standard operating procedure (SOP), the examination of the recalcification period of the citrate blood centrifuge is 20 minutes at a speed of 3000 rpm. Based on the facts in the field, samples were taken simultaneously, and several different examinations were carried out so that when the centrifuge was carried out, it was not by the SOP. So that there can be an error in the production of the examined citrate plasma. The study aimed to determine the effect of centrifuge time variation on the value of the recalcification period. The research method uses a quantitative approach, experimental research, and a cross-sectional research design. The sampling technique is Accidental Sampling, using nine samples from 9 patients with 3 treatments. The results of the data normality test with the Shapiro-Wilk test from the test results carried out can be seen in 3 data groups; from the data obtained, there is one abnormal treatment. So, the Kruskal-Wallis Test was continued, and the Kruskal-Wallis test was non-parametric. The conclusion obtained from the study is that there is an influence on the recalcification period.

References

[1] Aditea E. P, Yulia N.I and Yetti He. 2020. https://unair.ac.id/rekomendasi inspection plasma prothrombin time And activatepartial thromboplastin time. Accessed on March 15, 2024.
[2] Ady Supriad i. 2017. Freezing Mechanisms Blood. https://www.skokul.com/1804/mekanisme-pembekuan-darah/. ( On line ). Accessed August 17, 2024.
[3] Aghniya, MBC 2018. Differences in Serum Centrifuge Centrifugation Time. Muhammadiyah University Semarang.
[4] Aliviameita, A., & Puspitasari. 2019. Textbook of Hematology. Umsida Press, 1-56.
[5] Anto, 2019 PTT Lab Results Tall. https://www.alodokter.com/komunitas/topic/hasil-lab-prothrombin-dan-aptt-tinggi.
[6] Batavialab . 2017. Using good centrifugation. https://batavialab.com/berita/detail/usingcentrifuge - yang Good 27723.html.
[7] Bunjevac, A., Gabaj, N.A., Miler, M., Horvat, A. 2018. Preanalytics of urine sediment examination: effect of relative centrifugal force, tube type, volume of sample and supernatant removal. BiochemMed (Zagreb). doi:10.11613/BM.2018.010707.PMCID: PMC5806615.MID: 29472802.
[8] Dr. Pittara . 2022. Hemophilia. https://www.alodokter.com/hemophilia. (On line). Accessed August 15, 2024 .
[9] Duracim, A . 2018 . Hemostasis, Human Resources Education Center , Jakarta
[10] Eissa, RA; Saafan, HA; Ali, AE; Ibrahim, KM; Eissa, NG; Hamad, MA; Pang, C.; Guo, H.; Gao, H.; Elsabahy, M.; et al. Nanoconstruct design that exhibits increased hemostatic efficiency and bioabsorbability. Nanoscale 2022 , 14 , 10738–10749.
[11] Gruen, R.L.; Brohi, K.; Schreiber, M.; Balogh, Z.J.; Pitt, V.; Narayan, M.; Maier, Control of RV Hemorrhage in the critically injured patient. Lancet 2012 , 380 , 1099–10108.
[12] Kim Lipi. 2016 . Calibration definition, metrology research center, Tangerang. Accessed June 6, 2024).
[13] Mahara, IHA . 2018. Differences in Recalcification Period Examination Results Using Citrated Plasma Which Are Immediately Checked and Delayed at Temperature Space (23c). Https://repository.poltekkes smg.ac.id/index.php?p=show_detail&id=15434&keywords= (on line). Accessed July 12, 2024.
[14] Pratiwi DT. 2016. The effect of centrifugation time on the recalcification period. KTI. UNIMUS.
[15] Rosdianti, 2017. Effect of Centrifugation Time on Aptt Values (activated Partial Thromboplastin Time). (http://repository.unimus.ac.id/1275/Accessed on April 15, 24).
[16] Simamora, M. (2021). Recalcification. https://id.scribd.com/document/536843627/Mariane-Recalcification. (On line). Accessed July 12, 2024.
[17] Smith SA, Travers RJ, Morrissey JH. How it all starts: Initiation of the clotting cascade. Crit Rev Biochemistry Mol Biol. 2015; 50(4):326-36.
[18] Sreejith. N., (2023). Freezing. https://teachmephysiology.com/immune-system/haematology/coagulation/. (On line). Accessed July 14, 2024.
[19] Wang, XX; Liu, Q.; Sui, JX; Ramakrishna, S.; Yu, M.; Zhou, Y.; Jiang, XY; Long, YZ Recent Advances in Hemostasis at the Nanoscale. Adv. Health c. Material. 2019 , 8 , 1900823 .
[20] Wiarsih S. 2018. Differences in Recalcification Period Examination Results on Variations in Spinning Speed. Scientific papers . Semarang Ministry of Health Polytechnic .
[21] Wiarsih, S., 2018. Differences in Recalcification Period Examination Results in Variations Spinning Speed. Https://Repository.Poltekkes smg.ac.id/index.php? p=show_detail&id=15515& keywords =. (on line). Accessed July 12, 2024.
[22] Yani, RA . 2019. Various Components of Human Blood. https://rsisurabaya.com/cepatkompondarah human/#:~:text=So%20in%20as a whole%2C%20components%20of blood,%20blood%20white%2C%20cells and%20platelets. (On line). Accessed July 13, 2024.
[23] Yulianti, C., 202 2 . 7 Functions of Blood for the Body and Characteristics. https://www.detik.com/edu/detikpedia/d-6422709/7-function-darah-bagi-body as well as-characteristics Which must student know. (On line). Accessed July 13, 2024.
[24] Smith SA, Travers RJ, Morrissey JH. How it all starts: Initiation of the clotting cascade. Crit Rev Biochemistry Mol Biol. 2015; 50(4):326-36.
[25] Sreejith. N., (2023). Freezing. https://teachmephysiology.com/immune-system/haematology/coagulation/. (On line). Accessed July 14, 2024.
[26] Wang, XX; Liu, Q.; Sui, JX; Ramakrishna, S.; Yu, M.; Zhou, Y.; Jiang, XY; Long, YZ Recent Advances in Hemostasis at the Nanoscale. Adv. Health c. Material. 2019 , 8 , 1900823 .
[27] Wiarsih S. 2018. Differences in Recalcification Period Examination Results on Variations in Spinning Speed. Scientific papers . Semarang Ministry of Health Polytechnic .
[28] Wiarsih, S., 2018. Differences in Recalcification Period Examination Results in Variations Spinning Speed. Https://Repository.Poltekkes smg.ac.id/index.php?p=show_detail&id=15515& keywords =. (on line). Accessed July 12, 2024.
[29] Yani, RA . 2019. Various Components of Human Blood. https://rsisurabaya.com/cepatkompondarah human/#:~:text=So%20in%20as a whole%2C%20components%20of blood,%20blood%20white%2C%20cells and%20platelets. (On line). Accessed July 13, 2024.
[30] Yulianti, C., 202 2 . 7 Functions of Blood for the Body and Characteristics. https://www.detik.com/edu/detikpedia/d-6422709/7-function-darah-bagi-body as well as-characteristics Which must student know. (On line). Accessed July 13, 2024.