Journal of Health, Technology and Science (JHTS)

DETECTION OF Staphylococcus aureus BACTERIA ON ESCALATOR HANDRAINS IN GORONTALO SHOPPING CENTER

dheyiska tahir, Yolan Dunggio, Diah Astini Paramita — Thu, 20 Jun 2024 00:00:00 +0000

Studies have shown that various types of bacteria can be found on escalator handrails, including Staphylococcus aureus . This bacteria can survive and reproduce on the surface of the escalator handrail, especially if environmental conditions allow, such as the right humidity and temperature. The purpose of this study was to determine the detection of bacteriaStaphylococcus aureus on escalator handrails .The method in this study uses a qualitative approach with a descriptive research type. This study was conducted using the scratch method or sample culture on Mannitol media. Salt Agar (MSA) and macroscopic , microscopic and catalase biochemical tests were carried out. The results of the study from 8 samples showed that 5 samples detected Staphylococcus aureus bacteria macroscopically showed round, irregular round, yellow, yellowish white and mucoid slimy colonies. Microscopic results showed round, grape-shaped, clustered and purple bacteria which were gram-positive bacteria.In the biochemical test (catalase test) gas bubbles were formed . While 3 samples did not detect Staphylococcus aureus bacteria by showing irregular colonies, yellowish white in color, microscopic results of rod-shaped bacteria (Bacillus), gram positive, in the Biochemical test (catalase) no air bubbles were formed.

COMPARISON OF TOTAL BILIRUBIN LEVELS IN SERUM WITHOUT LIGHT EXPOSURE DELAYED FOR 1 HOUR AND 3 HOURS

niluh yuniwarsiti, Rizman Naim, Laras Puspita Taib — Thu, 20 Jun 2024 00:00:00 +0000

The total bilirubin test is one of the laboratory tests to determine the function of the liver and bile ducts. Impaired liver function can be shown by hemolytic anemia, liver cirrhosis, hepatitis, and hepatitis carcinoma. This study aims to determine the comparison of total bilirubin levels in serum without light exposure that is delayed for 1 hour and 3 hours. The method in this study uses a quantitative approach with a type of experimental research of Cross sectional design. The types of data used are primary data and secondary data. The sampling technique uses the purposive sampling technique, with a sample number of 10 samples. The results showed that the average value of total bilirubin examination in serum (immediately checked) with an average result value of 0.514 mg/dL, while in serum it was delayed for 1 hour with an average result of 0.513 mg/dL and serum was delayed for 3 hours with an average value of 0.511 mg/dL and the result of the value (significance) > from alpha (0.05) or (sig > 0.05) then Ho was accepted and Ha was rejected which means There was no difference in the results of total bilirubin levels in serum without light exposure that was delayed by 1 hour and 3 hours.

DESCRIPTION OF TRIGLYCERIDE LEVELS IN MEN WHO CONSUME ALCOHOLIC BEVERAGES IN BONGOIME VILLAGE,TILONGKABILA DISTRICT, BONE BOLANGOREGENCY, GORONTALO PROVINCE

meylan bagu, Laksmyn Kadir, Rizman Naim — Thu, 20 Jun 2024 00:00:00 +0000

Alcohol consumption is often associated with increased plasma triglyceride concentrations and has relevance to the risk of cardiovascular disease and pancreatitis. Ethanol consumed, as much as 90%, will be metabolized by the body, especially in the liver. Ethanol metabolism in the liver results in an increase in cytosolic and mitochondrial nicotinamide adenine dinucleotide dehydrogenase (NADH), leading to disruption of normal metabolism in the liver. The purpose of this study is to find out the picture of thyrglyceride levels in men who consume alcoholic beverages.The method in this study uses a quantitative approach with a descriptive type of research. The sampling technique in this study uses a purposive sampling technique, assuming the number of samples is 20 samples. Using univariate data analysis techniques.The results of the study showed that in people who consumed alcohol seen from the frequency distribution based on age, at the age of 20-30 years had a thyricide level of (35%), at the age of 31-40 years there was a total (45%), at the age of 41-50 there was a total (10%), and 51-60 a total (10%), the results showed that the most presentation was at the age of 31-40 years with a percentage (45%) based on the results of triglyceride tests in alcohol consumers, Normal results were obtained from 13 respondents and abnormal results for 7 respondents, with an average normal result of 105 mg/dl and an average abnormal result of 231 mg/dl and there was an influence on the number of alcohol consumers with triglyceride levels.

IDENTIFICATION OF MYCOBACTERIUM LEPRAE IN HOUSEHOLD CONTACTS OF LEPROSY PATIENTS IN BONE BOLANGO DISTRICT

Putri Sangit, Yolan Dunggio, Nasar — Thu, 20 Jun 2024 00:00:00 +0000

The purpose of this study was to identify the presence of Mycobacterium leprae and determine the bacterial index (IB) and morphological index (IM) in household contacts of leprosy patients in Bone Bolango Regency. The research method used a descriptive approach with data collection methods through observation, interviews, and laboratory examinations. Samples were taken from household contacts of leprosy patients in two sub-districts with the highest number of new leprosy cases, namely Bone Raya District and Tapa District. The examination was carried out in the laboratory of Toto Kabila Hospital. The results of the study showed that of the five samples studied, one sample showed positive results with a bacterial index (IB) of +3, which means that there were 1-10 acid-fast bacteria (AFB) in one field of view. The morphological index (IM) of the positive sample was 50%. These results indicate the transmission of M. leprae among household contacts of leprosy patients. Conclusion : There is One case positive of the five samples tested . The samples own index bacteria (IB) +3, which indicates the presence of 1-10 BTA germs in One roomy view . On the index morphological (IM) found by 50%. Findings This emphasize importance routine check and detection early For prevent distribution disease leprosy.

CENTRIFUGE TIME VARIATIONS ON RECALCIFICATION PERIOD VALUE

Fathan Mantu, Rita Amini Warastuti, Marlia — Thu, 20 Jun 2024 00:00:00 +0000

Hemostasis is the body’s mechanism to stop bleeding during an injury. The recalcification period examination is a specific hematological examination of blood clotting disorders to look for deficiency of clotting factors from the intrinsic pathway (Factors V, VIII, IX, X, XI, and XII), prothrombin and fibrinogen. According to the standard operating procedure (SOP), the examination of the recalcification period of the citrate blood centrifuge is 20 minutes at a speed of 3000 rpm. Based on the facts in the field, samples were taken simultaneously, and several different examinations were carried out so that when the centrifuge was carried out, it was not by the SOP. So that there can be an error in the production of the examined citrate plasma. The study aimed to determine the effect of centrifuge time variation on the value of the recalcification period. The research method uses a quantitative approach, experimental research, and a cross-sectional research design. The sampling technique is Accidental Sampling, using nine samples from 9 patients with 3 treatments. The results of the data normality test with the Shapiro-Wilk test from the test results carried out can be seen in 3 data groups; from the data obtained, there is one abnormal treatment. So, the Kruskal-Wallis Test was continued, and the Kruskal-Wallis test was non-parametric. The conclusion obtained from the study is that there is an influence on the recalcification period.