ANALYSIS OF THE RESULTS OF THE ABO BLOOD BLOOD CLASS EXAMINATION WITH ANTISERA REAGENT IS ENROLLMENT AND NOT DECLARATED AT THE OTANAHA GORONTALO Hospital

Abstract

Blood is part of the body's transport system which consists of two parts, namely blood plasma and blood cells consisting of erythrocytes, leukocytes, and platelets. One of the blood tests is the determination of the type of blood by looking at the degree of agglutination. The effect of diluting the antisera reagent on agglutination is not yet known. This study aims to see the differences in the results of agglutination in the ABO system blood group examination with diluted and undiluted antisera reagents.

This type of research is quantitative research with a quasy experimental design. The sampling technique was purposive sampling with a total sample size of 20 experimental respondents (50%, 40%, and 20% Antisera dilution) and control (without dilution) and then compared the experimental and control results using the comparative non-parametric Kruskall-Wallis test. (α = 5%).

The results of this study are that there is a significant difference (0.00 <0.05) between the experimental and control groups (0.00 <0.05) and there is a significant effect (H hit> H table) between the experimental and control groups (151,674> 77.930) So it can be concluded that there is a significant difference and influence on the analysis of the results of the ABO system blood group examination agglutination with diluted and undiluted antisera reagents. The decrease in the degree of agglutination from the results of the blood group examination is caused by the higher dilution, which affects the antibody ratio in the antisera.