DETERMINATION OF TOTAL FLAVONOID LEVELS OF KAFFIR LIME (Citrus hystrix) LEAF EXTRACT USING SOXHLETATION METHOD USING UV-VIS SPECTROPHOTOMETER

Abstract

Kaffir lime (Citrus histrix) is one of the plants with abundant flavonoid content in the leaves. The characteristic flavonoids with the two benzene ring groups cause the process of finding an appropriate extraction technique.

This study aims to determine the total flavonoid levels of kaffir lime leaf extract obtained from the soxhletation extraction method. This research was carried out by extracting simplicia kaffir lime leaves by the soxhletation method using 96% ethanol solvent. Comparison between the simplicia and the solvent used is 1: 5, then the extract obtained was carried out with initial qualitative identification of flavonoids and total flavonoid levels were determined using UV-Vis spectrophotometer.

The results showed that the extract of kaffir lime leaves obtained by soxhletation extraction in qualitative and quantitative tests contained flavonoid compounds with a total content of 5.62%