THE EFFECT OF CHILDREN (Ocimum sanctum) LEAF EXTRACT ON BACTERIA GROWTH Staphylococcus aureus

  • Mohammad Andi Laboddu Universitas Bina Mandiri Gorontalo
Keywords: Bahasa Indonesia

Abstract

       This research aims todetermine the effect of basil leaf extract (Ocinum sanctum) on the growth of Staphylococcus aureus and the optimal concentration of basil leaf extract (Ocinum sanctum) in inhibiting the growth of Staphylococcus aureus.

       This research method used a completely randomized design (CRD) with 5 treatments with 4 replications consisting of negative control (aquades), positive control (Amoxicillin), extract 20%, 40% and 60%.

       Research results obtained that basil leaf extract (Ocinum sanctum) has an effect on the growth of Staphylococcus aureus bacteria. The optimal concentration of basil leaf extract (Ocinum sanctum) in inhibiting the growth of Staphylococcus aureus bacteria is at a concentration of 40% with the category of "strong" bacterial inhibition.

 

Keywords :Basil (Ocinum sanctum), Staphylococcus aureus, Extract, Bacteria

References

[1] Adeola, SA, Folorunso, OS, & Amisu, KO, 2012, Antimicrobial Activity Ocimum basilicum and its Inhibition on the Characterized and Partially Purified Extracellular Protease of Salmonella typhimurium, Research Journal of Biology, 2 (5), 138-144.
[2] Angelina, M., Turnip, M. Khotimah, S. 2015. Antibacterial Activity Test of Basil (Ocinum sanctum) Leaf Ethanol Extract Against Bacterial Growth Escherichia coli and Staphylococcus aureus. Protobiont Journal, Tanjungpura University.
[3] Atikah, N. 2013. Antimicrobial Activity Test of Basil Herb Extract (Ocimum ladanum L) against Staphylococcus aureus and Candida albicans. Essay. Jakarta: UIN Syarif Hidayatullah Jakarta.
[4] Agusta, A. 2000. Indonesian tropical plant essential oils, 5,9,18-19,23,89. ITB: Bandung
[5] Baind-Parker, TC. 2000. Da;a, Beuchat :R. 1994, Staphylococcus Species; Antimicrobial Properties of Species and Theirs Essential Oils: BM. Lund, TC Braind-Parker and GW Goulds (Eds). The Microbiology Safety and Quality of Food. Aspen Publ Inc, Maryland
[5] Brooks, GF, Janet, SB, Stephen AM 2007. Jawetz, Melnick and Adelbergs,
Medical Microbiology Edition 23, Translated by Mudihardi, E., Kuntaman, Wasito, EB, Mertaniasih, NM, Harsono, S., and
Alimsardjono, L. Jakarta: EGC Medical Book Publisher. pp. 163, 170, 225-31, 253.
[6] Chandra, 2011. Basil Maintains Reproductive Health. http://www.tribunnews.com/2011/09/28/kemangi-kemenjaga-reproductive health. [January 24, 2014].
[7] Davis, WW and Stout, TR 1971. Disc Plate Method of Microbiological Antibiotic Assay. Applied Microbiology. 22(4): 659-665.
[8] Directorate General of POM. 1986. Galenic Preparations. Indonesian Ministry of Health: Jakarta
[9] Dhulgande, G., ARBirari & DADhale. 2010. Preliminary Screening of Antibacterial and Phytochemical Studies of Ocimum americanum Linn. Journal of Ecobiotechnology, 2 (8) : 11-13
[10] Nice Azkawati. 2016. Antibacterial Activity of Basil Leaf Extract (Ocimum Sanctum) Against Methicilin Resistant Staphylococcus Aureus. Health Polytechnic. Bandung
[11] Fitriani Tallama. (2014). The effectiveness of basil leaf extract on the reduction of
levels of volatile sulfur compounds(VSCs) Obtained solid on November 10, 2017.
[12] FKUI., 2002. Textbook of Medical MicrobiologyRevised Edition. Character Building. Jakarta
[13] Gibson, J.M., 1996, Modern Microbiology and Pathology for Nursing, 12 EGC Medical Book Publisher, Jakarta.
[14] Ganiswarna, SG, 2007, Pharmacology and Therapy 5th Edition, Department of Pharmacology and Therapeutics, Faculty of Medicine UI, Jakarta
[15] Handayani, PA & M. Safaatul. 2010. Extraction of Kaffir lime leaf oil (Citrus hystrix DC) with ethanol and N-hexane as solvents. Journal
Technical Competence. 2 (1).
[16] Jawetz, E., Melnick, JL, Adelberg, EA, 1996, Medical Microbiology, 20th Edition, 213, EGC, Medical Book Publisher, Jakarta
[17] Kusuma, W. 2010. Effects of basil leaf extract (Ocinum sanctum L.)
damage to hepatocytes of mice due to palm oil with repeated heating. Essay. Faculty of Medicine, Sebelas Maret University: Surakarta
[18] Maida, S., Lestari, PAK 2019. Amoxicillin Antibacterial Activity Against Gram Positive and Gram Negative Bacteria. J. Pijar MIPA Vol. 14 No. 3. Surabaya Pharmacy Academy
[19] Mardia. 2017. Test of Staphylococcus aureus Resistance to Antibiotics
Amoxcillin, Tetracycline and Propolis. Muhammadiyah Academy of Health Analyst. Makassar
[20] Moeza, KM 2019. Inhibitory Test of Basil Leaf Extract (Ocinum citriodorum Vis) Against the Growth of Escherichia Coli Bacteria. University of Northern Sumatra. Medan.
Published
2023-11-21
How to Cite
LabodduM. A. (2023). THE EFFECT OF CHILDREN (Ocimum sanctum) LEAF EXTRACT ON BACTERIA GROWTH Staphylococcus aureus. Journal of Health, Technology and Science (JHTS), 4(3), 121-131. https://doi.org/10.47918/jhts.v4i3.437