THE EFFECT OF CHILDREN (Ocimum sanctum) LEAF EXTRACT ON BACTERIA GROWTH Staphylococcus aureus
Abstrak
Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun kemangi (Ocinum sanctum) terhadap pertumbuhan Staphylococcus aureus serta konsentrasi optimalekstrak daun kemangi (Ocinum sanctum) dalam menghambat pertumbuhan Staphylococcus aureus.
Metode penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan 4 kali ulangan yang terdiri dari kontrol negative (aquades), kontrol positif (Amoxicillin), Ekstrak 20%, 40% dan 60%.
Hasil penelitian diperoleh bahwa ekstrak daun kemangi (Ocinum sanctum) memiliki pengaruh terhadap pertumbuhan bakteri Staphylococcus aureus. Konsentrasi optimal ekstrak daun kemangi (Ocinum sanctum) dalam meghambat pertumbuhan bakteri Staphylococcus aureus yaitu pada konsentrasi 40% dengan kategori daya hambat bakteri “kuat”.
Kata Kunci : Kemangi (Ocinum sanctum), Staphylococcus aureus, Ekstrak, Bakteri
Referensi
[1] Adeola, SA, Folorunso, OS, & Amisu, KO, 2012, Antimicrobial Activity Ocimum basilicum and its Inhibition on the Characterized and Partially Purified Extracellular Protease of Salmonella typhimurium, Research Journal of Biology, 2 (5), 138-144.
[2] Angelina, M., Turnip, M. Khotimah, S. 2015. Antibacterial Activity Test of Basil (Ocinum sanctum) Leaf Ethanol Extract Against Bacterial Growth Escherichia coli and Staphylococcus aureus. Protobiont Journal, Tanjungpura University.
[3] Atikah, N. 2013. Antimicrobial Activity Test of Basil Herb Extract (Ocimum ladanum L) against Staphylococcus aureus and Candida albicans. Essay. Jakarta: UIN Syarif Hidayatullah Jakarta.
[4] Agusta, A. 2000. Indonesian tropical plant essential oils, 5,9,18-19,23,89. ITB: Bandung
[5] Baind-Parker, TC. 2000. Da;a, Beuchat :R. 1994, Staphylococcus Species; Antimicrobial Properties of Species and Theirs Essential Oils: BM. Lund, TC Braind-Parker and GW Goulds (Eds). The Microbiology Safety and Quality of Food. Aspen Publ Inc, Maryland
[5] Brooks, GF, Janet, SB, Stephen AM 2007. Jawetz, Melnick and Adelbergs,
Medical Microbiology Edition 23, Translated by Mudihardi, E., Kuntaman, Wasito, EB, Mertaniasih, NM, Harsono, S., and
Alimsardjono, L. Jakarta: EGC Medical Book Publisher. pp. 163, 170, 225-31, 253.
[6] Chandra, 2011. Basil Maintains Reproductive Health. http://www.tribunnews.com/2011/09/28/kemangi-kemenjaga-reproductive health. [January 24, 2014].
[7] Davis, WW and Stout, TR 1971. Disc Plate Method of Microbiological Antibiotic Assay. Applied Microbiology. 22(4): 659-665.
[8] Directorate General of POM. 1986. Galenic Preparations. Indonesian Ministry of Health: Jakarta
[9] Dhulgande, G., ARBirari & DADhale. 2010. Preliminary Screening of Antibacterial and Phytochemical Studies of Ocimum americanum Linn. Journal of Ecobiotechnology, 2 (8) : 11-13
[10] Nice Azkawati. 2016. Antibacterial Activity of Basil Leaf Extract (Ocimum Sanctum) Against Methicilin Resistant Staphylococcus Aureus. Health Polytechnic. Bandung
[11] Fitriani Tallama. (2014). The effectiveness of basil leaf extract on the reduction of
levels of volatile sulfur compounds(VSCs) Obtained solid on November 10, 2017.
[12] FKUI., 2002. Textbook of Medical MicrobiologyRevised Edition. Character Building. Jakarta
[13] Gibson, J.M., 1996, Modern Microbiology and Pathology for Nursing, 12 EGC Medical Book Publisher, Jakarta.
[14] Ganiswarna, SG, 2007, Pharmacology and Therapy 5th Edition, Department of Pharmacology and Therapeutics, Faculty of Medicine UI, Jakarta
[15] Handayani, PA & M. Safaatul. 2010. Extraction of Kaffir lime leaf oil (Citrus hystrix DC) with ethanol and N-hexane as solvents. Journal
Technical Competence. 2 (1).
[16] Jawetz, E., Melnick, JL, Adelberg, EA, 1996, Medical Microbiology, 20th Edition, 213, EGC, Medical Book Publisher, Jakarta
[17] Kusuma, W. 2010. Effects of basil leaf extract (Ocinum sanctum L.)
damage to hepatocytes of mice due to palm oil with repeated heating. Essay. Faculty of Medicine, Sebelas Maret University: Surakarta
[18] Maida, S., Lestari, PAK 2019. Amoxicillin Antibacterial Activity Against Gram Positive and Gram Negative Bacteria. J. Pijar MIPA Vol. 14 No. 3. Surabaya Pharmacy Academy
[19] Mardia. 2017. Test of Staphylococcus aureus Resistance to Antibiotics
Amoxcillin, Tetracycline and Propolis. Muhammadiyah Academy of Health Analyst. Makassar
[20] Moeza, KM 2019. Inhibitory Test of Basil Leaf Extract (Ocinum citriodorum Vis) Against the Growth of Escherichia Coli Bacteria. University of Northern Sumatra. Medan.
[2] Angelina, M., Turnip, M. Khotimah, S. 2015. Antibacterial Activity Test of Basil (Ocinum sanctum) Leaf Ethanol Extract Against Bacterial Growth Escherichia coli and Staphylococcus aureus. Protobiont Journal, Tanjungpura University.
[3] Atikah, N. 2013. Antimicrobial Activity Test of Basil Herb Extract (Ocimum ladanum L) against Staphylococcus aureus and Candida albicans. Essay. Jakarta: UIN Syarif Hidayatullah Jakarta.
[4] Agusta, A. 2000. Indonesian tropical plant essential oils, 5,9,18-19,23,89. ITB: Bandung
[5] Baind-Parker, TC. 2000. Da;a, Beuchat :R. 1994, Staphylococcus Species; Antimicrobial Properties of Species and Theirs Essential Oils: BM. Lund, TC Braind-Parker and GW Goulds (Eds). The Microbiology Safety and Quality of Food. Aspen Publ Inc, Maryland
[5] Brooks, GF, Janet, SB, Stephen AM 2007. Jawetz, Melnick and Adelbergs,
Medical Microbiology Edition 23, Translated by Mudihardi, E., Kuntaman, Wasito, EB, Mertaniasih, NM, Harsono, S., and
Alimsardjono, L. Jakarta: EGC Medical Book Publisher. pp. 163, 170, 225-31, 253.
[6] Chandra, 2011. Basil Maintains Reproductive Health. http://www.tribunnews.com/2011/09/28/kemangi-kemenjaga-reproductive health. [January 24, 2014].
[7] Davis, WW and Stout, TR 1971. Disc Plate Method of Microbiological Antibiotic Assay. Applied Microbiology. 22(4): 659-665.
[8] Directorate General of POM. 1986. Galenic Preparations. Indonesian Ministry of Health: Jakarta
[9] Dhulgande, G., ARBirari & DADhale. 2010. Preliminary Screening of Antibacterial and Phytochemical Studies of Ocimum americanum Linn. Journal of Ecobiotechnology, 2 (8) : 11-13
[10] Nice Azkawati. 2016. Antibacterial Activity of Basil Leaf Extract (Ocimum Sanctum) Against Methicilin Resistant Staphylococcus Aureus. Health Polytechnic. Bandung
[11] Fitriani Tallama. (2014). The effectiveness of basil leaf extract on the reduction of
levels of volatile sulfur compounds(VSCs) Obtained solid on November 10, 2017.
[12] FKUI., 2002. Textbook of Medical MicrobiologyRevised Edition. Character Building. Jakarta
[13] Gibson, J.M., 1996, Modern Microbiology and Pathology for Nursing, 12 EGC Medical Book Publisher, Jakarta.
[14] Ganiswarna, SG, 2007, Pharmacology and Therapy 5th Edition, Department of Pharmacology and Therapeutics, Faculty of Medicine UI, Jakarta
[15] Handayani, PA & M. Safaatul. 2010. Extraction of Kaffir lime leaf oil (Citrus hystrix DC) with ethanol and N-hexane as solvents. Journal
Technical Competence. 2 (1).
[16] Jawetz, E., Melnick, JL, Adelberg, EA, 1996, Medical Microbiology, 20th Edition, 213, EGC, Medical Book Publisher, Jakarta
[17] Kusuma, W. 2010. Effects of basil leaf extract (Ocinum sanctum L.)
damage to hepatocytes of mice due to palm oil with repeated heating. Essay. Faculty of Medicine, Sebelas Maret University: Surakarta
[18] Maida, S., Lestari, PAK 2019. Amoxicillin Antibacterial Activity Against Gram Positive and Gram Negative Bacteria. J. Pijar MIPA Vol. 14 No. 3. Surabaya Pharmacy Academy
[19] Mardia. 2017. Test of Staphylococcus aureus Resistance to Antibiotics
Amoxcillin, Tetracycline and Propolis. Muhammadiyah Academy of Health Analyst. Makassar
[20] Moeza, KM 2019. Inhibitory Test of Basil Leaf Extract (Ocinum citriodorum Vis) Against the Growth of Escherichia Coli Bacteria. University of Northern Sumatra. Medan.
Diterbitkan
2023-11-21
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LabodduM. A. (2023). THE EFFECT OF CHILDREN (Ocimum sanctum) LEAF EXTRACT ON BACTERIA GROWTH Staphylococcus aureus. Journal of Health, Technology and Science (JHTS), 4(3), 121-131. https://doi.org/10.47918/jhts.v4i3.437
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