TEST OF PREPARATION FORMULATION SPRAY MANGROVE LEAF EXTRACT RHIZOPORA MUCRONATA AS REPELLENT AGAINST MOSQUITO ANOPHELES VAGUS
Abstract
The mangrove plant Rhizopora mucronata is a plant that belongs to the Family Rhizophoraceae and is often found in tidal areas, one of which is in Boalemo Regency, Bajo Village. This plant is used as a repellent on the leaves. The secondary metabolite compounds contained in the leaves of Rhizopora mucronata function as repellent namely alkaloids, flavonoids, tannins and terpenoids. This study aims to determine the activity and significant differences in the concentration of the preparation formulation spray mangrove leaf extract Rhizopora mucronata as repellent against mosquitoes Anopheles vagus. Laboratory experimental research method with the research object being mangrove leaf extract Rhizopora mucronata which includes the maceration and extraction stages with methanol solvent, identification of secondary metabolite compounds, preparation evaluation test, and repellent test with 6 test groups, namely negative control, F0%, F10%, F15%, F20%, and positive control (soffel spray ). The results of the study showed that the formulation spray mangrove leaf extract Rhizopora mucronata had activity against mosquitoes Anopheles vagus due to mangrove leaf extract Rhizopora mucronata contains alkaloids, tannins, flavonoids and terpenoids which function as repellent . The preparation formulation spray mangrove leaf extract Rhizopora mucronata concentrations of 10%, 15%, and 20% do not have a significant difference in effect on mosquitoes Anopheles vagus, because at this concentration the compound content of mangrove leaves Rhizopora mucronata is equally effective as repellent against mosquitoes Anopheles vagus.
References
[2] Anita, A. S., Aristhasari, P., & Refida, C. 2021. Analysis of Tannin Content from Flower Petals of Rosella Red (Hibiscus sabdariffa, L. ) Permanganometry . Urecol Journal Part D Vol.1, No.1.
[3] Dila, G., Muhammad, I. K., & Ari, W. 2020. Effectiveness of Methanol Extract of Pandan Wangi Root ( Pandanus amaryllifolius Roxb . ) as an antimalarial on the number of parasitemia and monocytes in the blood of mice ( Mus musculus ) which is infected with Plasmodium berghei . Tanjungpura University, Pontianak.
[4] Endah, S. 2020. Getting to know Malaria and its vectors. Ali Imron Library, Lampung.
[5] Indah, S., Diah, A. S., & Tony, A. W. 2020. Phytochemical Screening for Secondary Metabolite Compounds of Dragon Fruit Stem (Hylocereus polyrhizus) . College of Pharmaceutical Sciences, Semarang.
[6] Irfayanti NA , Jasmiadi J, Tari A. Formulation and Activity Test of Repellent Spray Marigold Flower Essential Oil ( Tagetes erecta L. ) in Aedes aegypti Mosquitoes. 2022 May 25 4(2):363–70.
[7] Ramli, A., Muhammad, T., Naufal, N., & Daud, M. 2023. Comparison of the Yield of Production Process for Chinese Silkworm Thread and F1 Silkworms, Sabbeta Village, Pising Village, Donri-Donri District, Soppeng Regency. Muhammadiyah University, Makassar.
[8] Salimi, A. Widiantoro, Bialangi, N., M.A. Mustapa, Y.K. and B. Situmeang. 20 20 . Antimalarial Activity and Phytochemical Analysis From Order ( Peperomia pellucida) Extract. Journal of Chemical Education , 8. Page. 183-187.
[9] Saliny Aiyer, K.G. Manju. 2019. Phytochemical screening in vitro antioxidant activity, cytotoxicity study using Brine shrimp and antimicrobial study of (Linn.) Acanthus ilicifolius leaves. Journal. Department of Zoology, Mar Ivanios College, Trivandrum: India , 4(5):654-658.
[10] Sengodan, K., Karthic, U., Vinothkumar, M., Manigandan, V., Kamaraj, C., Prabhakaran, V. S., & Patcharin, K. 2020. Larvicidal Enzyme Inhibition and Repellent Activity of Red Mangrove Rhizophora mucronata (Lam.) Leaf Extracts and Their Biomolecules against Three Medically Challenging Arthropod Vectors. Molecules 2020, 25, 3844.
[11] Suharti., Risa, K. P., & Ibnu S. 2024. Test of the Potential for Reducing Cholesterol Levels in Mice (Mus musculus) from infusion of cherry leaves (Muntingia calabura L.) Fresh and Simplicia. Pharmaceutical Scientific Journal, Vol. 4, no. 1
[12] Syafrina, L., Azira, N. P., Erwin, N., Nita, A. & Agus P. 2023. Larvicidal activity of the active fraction of black mangrove leaves Rhizophora mucronata Lamk. against mosquito larvae Aedes aegypti Linn. Journal of Science Research 25 (1) 2023: 25113(73-80.
[13] Tavares, M. 2018. Trends in insect repellent formulations : A review. International Journal of Pharmaceutics 539 (2018) 190-209.
[14] WHOPES. (2009). Guidelines for efficacy testing of mosquito repellents for human skin. In Who/Htm/Ntd/Whopes/2009.4.
Copyright (c) 2024 Nur'ain Dunda, Sucia Pratiwi Tampoy, Adnan Malaha

This work is licensed under a Creative Commons Attribution 4.0 International License.