TEST OF PREPARATION FORMULATION SPRAY MANGROVE LEAF EXTRACT RHIZOPORA MUCRONATA AS REPELLENT AGAINST MOSQUITO ANOPHELES VAGUS

  • Nur'ain Dunda Bina Mandiri University Gorontalo, Indonesia
  • Sucia Pratiwi Tampoy Bina Mandiri University Gorontalo, Indonesia
  • Adnan Malaha Bina Mandiri University Gorontalo, Indonesia
Keywords: Rhizopora mucronata, Repellent, Anopheles vagus

Abstract

The mangrove plant   Rhizopora mucronata  is a plant that belongs to the Family   Rhizophoraceae   and is often found in tidal areas, one of which is in Boalemo Regency, Bajo Village. This plant is used as a   repellent   on the leaves. The secondary metabolite compounds contained in the leaves of   Rhizopora mucronata   function as   repellent   namely alkaloids, flavonoids, tannins and terpenoids.    This study aims to determine the activity and significant differences in the concentration of the preparation formulation   spray   mangrove leaf extract   Rhizopora mucronata   as   repellent   against mosquitoes   Anopheles vagus.  Laboratory experimental research method with the research object being mangrove leaf extract   Rhizopora mucronata   which includes the maceration and extraction stages with methanol solvent, identification of secondary metabolite compounds, preparation evaluation test, and   repellent   test with 6 test groups, namely negative control, F0%, F10%, F15%, F20%, and positive control (soffel   spray  ).  The results of the study showed that the formulation   spray   mangrove leaf extract   Rhizopora mucronata   had activity against mosquitoes   Anopheles vagus  due to mangrove leaf extract   Rhizopora mucronata    contains alkaloids, tannins, flavonoids and terpenoids which function as   repellent  . The preparation formulation   spray   mangrove leaf extract   Rhizopora mucronata  concentrations of 10%, 15%, and 20% do not have a significant difference in effect on mosquitoes   Anopheles vagus, because     at this concentration the compound content of mangrove leaves   Rhizopora mucronata   is equally effective as   repellent   against mosquitoes   Anopheles vagus.

References

[1] Ali, R., Rini, P., Koesoemadji., Endang, S., Nirwani, S. 2017. Antioxidant activity of Rhizopora mucronata mangrove leaf extract. Marina Oceanographic Bulletin. 111.
[2] Anita, A. S., Aristhasari, P., & Refida, C. 2021. Analysis of Tannin Content from Flower Petals of Rosella Red (Hibiscus sabdariffa, L. ) Permanganometry . Urecol Journal Part D Vol.1, No.1.
[3] Dila, G., Muhammad, I. K., & Ari, W. 2020. Effectiveness of Methanol Extract of Pandan Wangi Root ( Pandanus amaryllifolius Roxb . ) as an antimalarial on the number of parasitemia and monocytes in the blood of mice ( Mus musculus ) which is infected with Plasmodium berghei . Tanjungpura University, Pontianak.
[4] Endah, S. 2020. Getting to know Malaria and its vectors. Ali Imron Library, Lampung.
[5] Indah, S., Diah, A. S., & Tony, A. W. 2020. Phytochemical Screening for Secondary Metabolite Compounds of Dragon Fruit Stem (Hylocereus polyrhizus) . College of Pharmaceutical Sciences, Semarang.
[6] Irfayanti NA , Jasmiadi J, Tari A. Formulation and Activity Test of Repellent Spray Marigold Flower Essential Oil ( Tagetes erecta L. ) in Aedes aegypti Mosquitoes. 2022 May 25 4(2):363–70.
[7] Ramli, A., Muhammad, T., Naufal, N., & Daud, M. 2023. Comparison of the Yield of Production Process for Chinese Silkworm Thread and F1 Silkworms, Sabbeta Village, Pising Village, Donri-Donri District, Soppeng Regency. Muhammadiyah University, Makassar.
[8] Salimi, A. Widiantoro, Bialangi, N., M.A. Mustapa, Y.K. and B. Situmeang. 20 20 . Antimalarial Activity and Phytochemical Analysis From Order ( Peperomia pellucida) Extract. Journal of Chemical Education , 8. Page. 183-187.
[9] Saliny Aiyer, K.G. Manju. 2019. Phytochemical screening in vitro antioxidant activity, cytotoxicity study using Brine shrimp and antimicrobial study of (Linn.) Acanthus ilicifolius leaves. Journal. Department of Zoology, Mar Ivanios College, Trivandrum: India , 4(5):654-658.
[10] Sengodan, K., Karthic, U., Vinothkumar, M., Manigandan, V., Kamaraj, C., Prabhakaran, V. S., & Patcharin, K. 2020. Larvicidal Enzyme Inhibition and Repellent Activity of Red Mangrove Rhizophora mucronata (Lam.) Leaf Extracts and Their Biomolecules against Three Medically Challenging Arthropod Vectors. Molecules 2020, 25, 3844.
[11] Suharti., Risa, K. P., & Ibnu S. 2024. Test of the Potential for Reducing Cholesterol Levels in Mice (Mus musculus) from infusion of cherry leaves (Muntingia calabura L.) Fresh and Simplicia. Pharmaceutical Scientific Journal, Vol. 4, no. 1
[12] Syafrina, L., Azira, N. P., Erwin, N., Nita, A. & Agus P. 2023. Larvicidal activity of the active fraction of black mangrove leaves Rhizophora mucronata Lamk. against mosquito larvae Aedes aegypti Linn. Journal of Science Research 25 (1) 2023: 25113(73-80.
[13] Tavares, M. 2018. Trends in insect repellent formulations : A review. International Journal of Pharmaceutics 539 (2018) 190-209.
[14] WHOPES. (2009). Guidelines for efficacy testing of mosquito repellents for human skin. In Who/Htm/Ntd/Whopes/2009.4.
Published
2024-09-27
How to Cite
DundaN., TampoyS. P., & MalahaA. (2024). TEST OF PREPARATION FORMULATION SPRAY MANGROVE LEAF EXTRACT RHIZOPORA MUCRONATA AS REPELLENT AGAINST MOSQUITO ANOPHELES VAGUS. Journal of Health, Technology and Science (JHTS), 5(3), 160-167. https://doi.org/10.47918/jhts.v5i3.2054