DETECTION OF Staphylococcus aureus BACTERIA ON ESCALATOR HANDRAINS IN GORONTALO SHOPPING CENTER
Abstrak
Studies have shown that various types of bacteria can be found on escalator handrails, including Staphylococcus aureus . This bacteria can survive and reproduce on the surface of the escalator handrail, especially if environmental conditions allow, such as the right humidity and temperature. The purpose of this study was to determine the detection of bacteriaStaphylococcus aureus on escalator handrails .The method in this study uses a qualitative approach with a descriptive research type. This study was conducted using the scratch method or sample culture on Mannitol media. Salt Agar (MSA) and macroscopic , microscopic and catalase biochemical tests were carried out. The results of the study from 8 samples showed that 5 samples detected Staphylococcus aureus bacteria macroscopically showed round, irregular round, yellow, yellowish white and mucoid slimy colonies. Microscopic results showed round, grape-shaped, clustered and purple bacteria which were gram-positive bacteria.In the biochemical test (catalase test) gas bubbles were formed . While 3 samples did not detect Staphylococcus aureus bacteria by showing irregular colonies, yellowish white in color, microscopic results of rod-shaped bacteria (Bacillus), gram positive, in the Biochemical test (catalase) no air bubbles were formed.
Referensi
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[2] Ampeni , I. S,202: Overview Bacteria Siaphylococus aureus On Student Mobile Phones : Medan.
[3] Aviany , HB, and Puiyanto , S, 2020. Analysis Effectiveness Probiotics in Products Beauty As Antibacterial To Staphylococcus epidermidis bacteria . Periodic Biotechnology , 3 (2 ).Becker , K., Heilmann, C., & Peters, G. (2019). Coagulase-Negative Staphylococci. Clinical Microbiology Reviews, 32(4), 00002-19.
[4] Cheung, GY, Joo, HS, Chatterjee, SS, & Otto, M. (2020). Phenol-soluble modulins —critical determinants of staphylococcal virulence. FEMS Microbiology Reviews , 44(6), 759-780.
[5] Chen, Y., Xu, C., Huang, Z., & Fang, Z. (2019). Metagenomic analysis of bacteria and antibiotic resistome from a metro station handrail. Environment international , 127, 732-741.
[6] Dewi, A. K, 2019. Isolation , Identification and Sensitivity Test Staphylococcus aureus Against Amoxicillin from Milk Samples of Ettawa Crossbred Goats (PE) Suffering from Mastitis in the Girimulyo Area , Kolonprogo , Yogyakarta . Journal of Veterinary Science , 31 (2), 138-150.
[7] Dewi, K., Asih, ENN, Fitri , DA, and Astutik , S, 2022. Detection physiological isolate bacteria halophilic from pool Selection people's salt ponds in the Regency Parnekasan . Juvenil: Scientific Journal of Marine and Fisheries, 3(3), 79-84.
[8] Foster, T. J., & Höök, M. (2021). Surface Protein Adhesins of Staphylococcus aureus. Trends in Microbiology , 29(1), 23-33.
[9] Foster, T. J. (2021). StaphylococcusIn Medical Microbiology (pp. 1-35). John Wiley & Sons, Ltd.
[10] Hayati, LN, Tyasningsih , W., Praja , RN, Chusniati , S., Yunita , MN, and Wibawati , P. A, 2019. Isolation and Identification Staphylococcus aureus in Etawah Crossbred Goat Milk Suffering from Subclinical Mastitis in the Sub-district Journal Medical Veterinary , 2 (2), 76-82.
[11] Janda, J.M., & Abbott, S.L. (2019). 16S rRNA Gene Sequencing for Bacterial Identification in the Diagnostic Laboratory: Pluses, Perils, and Pitfalls. Journal of Clinical Microbiology, 57(9), e00772-19.
[12] Jawetz , E., Adelberg , E.A., & Brooks , G.F. (2020). Jawetz , Melnick , & Adelberg's Medical Microbiology . Lange Medical Book / McGraw -Hill.
[13] Keliat, SP, Darniati, D., Harris, A., Erina, E., Rinidar , R., & Fahkrurrazi, F, 2019.
[14] 25. The effect of Fingerroot Rhizome ( Boesenbergia pandurata ) Extract on theGrowth of Staphylococcus aureus in Vitro . Journal of Veterinary Medicine , 13(2).
[15] Ministry of Health. (2022). Relationship between Knowledge, Attitude and Action of Nurses towards Prevention of Nosocomial Infection Incidents in the Internal Ward of Dr. M. Djamil Padang General Hospital in 2016. Padang: Andalas University.
[16] Kotay , S., Chai , WC, Kee , DHW, Alhorr , Y., & Ahmad, MF (2021). The Roles of Surface Materials on the Transmission of Pathogens in Healthcare Facilities : A Review . SN Comprehensive Clinical Medicine , 3(6), 2661-2672.
[17] Khairunnisa, M, 2018. Isolation and Identification of Staphylococcus aureus On the Udder
[18] 538-545. Etawa Crossbreed Goat (PE). Science Journal Veterinary Student , 2 (4), 538-545
[19] Kusumawardani, NP (2018). "Identification of Bacteria on Escalator Handrails in Several Malls in Jakarta". Thesis. Faculty of Public Health, University of Indonesia.
[20] Isolation and Identification of Staphylococcus aureus in Milk of Etawah Crossbred Goats Suffering from Subclinical Mastitis in Kalipuro Village, Banyuwangi. Faculty of Veterinary Medicine, PSDKU Banyuwangi, Airlangga University.
[21] Maimunah, S., Harefa, K., Yuliana, A., Ritonga, AH, and Hulu, A, 2019. ANTIBACTERIAL ACTIVITY TEST OF KERSEN LEAF EXTRACT (Muntingia calabura L.) AGAINST Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus BACTERIA. JOURNAL OF PHARMACY, 6 (2), 101-108.
[22] Mohan, D., & Padmanabhan, P. (2021). Identification of Staphylococcus aureus with Mannitol Salt Agar (MSA) and oxacillin resistance with Cefoxitin disk diffusion method in a tertiary care hospital, Tamil Nadu, India. Journal of Medical Science And Clinical Research , 9(1), 11-15.
[23] Poretsky, R., & Hugenholtz, P. (2019). Progress in the Study of Microbial Community Structure and Function through Metagenomics and Metatranscriptomics . Annual Review of Ecology, Evolution, and Systematics , 50, 201-227.
[24] Prananda, A. R, 2019. Identification of Bacteria in Grilled Meatballs, Sauce and Chili Sauce in Perwata Village, Teluk Betung Timur District. Research Article, J Agromedicine. Volume 6. Number 2.
[25] Radji, M. 2016. Microbiology Textbook Guide for Pharmacy and Medical Students. Medical Book. Jakarta: EGC.
[26] Redel, H., Chisomo-Kasiya, P., Loeb, M., & Johnstone, J. (2020). Detection of respiratory viruses on air filters from buses and high-touch area surfaces from urban schools. BMC Infectious Diseases , 20(1), 1-7.
[27] Riskesdas (2019). Basic Health Research 2019. Jakarta: Health Research and Development Agency, Ministry of the Republic of Indonesia.
[28] Rodriguez, A.M., & Gomez, N.N. (2019). " Staphylococcus aureus Contamination in Public Areas : A Case Study of Shopping Malls in Urban Settings ." International Journal of Hygiene and Environmental Health , 222(7), 1071-1080. doi:10.1016/j.ijheh.2019.06.005.
[29] Soedarto, 2015. Medical Microbiology. Jakarta: CV. Sagung Seto
[30] Tong, S.Y., Davis, J.S., Eichenberger , E., Holland , T.L., & Fowler Jr , V. G. (2021). Staphylococcus aureus infections : epidemiology , pathophysiology , clinical manifestations , and management . Clinical Microbiology Reviews , 28(3), 603-661.
[31] Turner, N.A., Sharma- Kuinkel , B.K., Maskarinec , S.A., Eichenberger , E.M., Shah, P.P., Carugati , M., Fowler Jr , V G ( 2019 ). Methicillin-resistant Staphylococcus aureus : an Overview of basic and clinical research . nature Reviews Microbiology , 17(4), 203-218.
[32] Völkening , J.D., Hennon , S.W., & Dworkin , J. (2020). Persistence : mechanisms for triggering and enhancement phenotypic variability . Current Genetics , 66(3), 423-429.
[33] Wertheim , HF, Melles , DC, Vos , MC, van Leeuwen , W., van Belkum , A., Verbrugh , H. A., & Nouwen , J. L. (2020). The roles of nasal carriage in Staphylococcus aureus infections . The Lancet Infectious Diseases , 5(12), 751-762.
[34] World Health Organization . (2019). First WHO Model List of Essential In Vitro
[35] Diagnostics : Volume 1017 (Vol. 1017). WorldHealth Organization .
Diterbitkan
2024-06-20
##submission.howToCite##
tahir dheyiska, DunggioY., & ParamitaD. A. (2024). DETECTION OF Staphylococcus aureus BACTERIA ON ESCALATOR HANDRAINS IN GORONTALO SHOPPING CENTER. Journal of Health, Technology and Science (JHTS), 5(2), 62-70. https://doi.org/10.47918/jhts.v5i2.1816
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