POTENTIAL OF ENDOPHY BACTERIAL ISOLATE FROM LEAF OF CHINESE TEMPERATURE (Cassia alata L) ON THE GROWTH OF THE BACTERIA OF STAPHYLOCOCCUS AUREUS

Enisa Kobandaha; Syam s kumaji; Adnan Malaha

doi:10.47918/jhts.v3i1.332

Enisa Kobandaha Universitas Bina Mandiri Gorontalo
Syam s kumaji
Adnan Malaha

DOI: https://doi.org/10.47918/jhts.v3i1.332

Abstrak

ABSTRACT

This research aims to To determine the effect of endophytic bacterial isolates from the leaves of the Chinese Ketepeng plant (Cassia Alata L) on the growth of Staphylococcus aureus and to determine the significant difference between each treatment of endophytic bacterial isolates from the leaves of the Ketepeng Cina (Cassia Alata L) plant against Staphylococcus aureus.

This research method used a completely randomized design (CRD) with 4 treatments and 6 replications. The treatments were: BEKC 1 (Chinese Ketepeng Endophytic Bacteria 1), BEKC 2 (Chinese Ketepeng Endophytic Bacteria 2), Positive Control (Chloramphenicol), and Negative Control (Aquades).

The results obtained two isolates of endophytic bacteria, namely isolates BEKC 1 with an inhibition zone of 9mm included in the medium category, and isolates BEKC 2 with an inhibition zone of 10mm included in the strong category. Analysis resultthat there was an effect of endophytic bacterial isolates from the leaves of the Chinese ketepeng plant (Cassia alata L) on the growth of Staphylococcus aureus bacteria. Then from the results of further tests using Duncan's test, it was found that each treatment, namely the negative control treatment was significantly different from BEKC 1, BEKC 2 and positive control, while there was no significant difference between BEKC 1, BEKC 2 and positive control.

Keywords : Endophytic Bacteria, Chinese Ketepeng (Cassia alata L), Staphylococcus aureus

Referensi

REFERENCES
[1] Abo KA, Adediwura AA, Ibikunle AJ. 1st International Workshop on Herbal Medicinal Products. University of Ibadan, Ibadan, Nigeria: 1998; 22 - 24. In: Makinde AA, Igoli JO, TA'Ama L, Shaibu SJ, Garba A. Antimicrobial activity of Cassia alata. Afr J. of Biotech. 2007; 6(13): 1509-10
[2] Arista, I, S. 2013. Isolation of Endophytic Fungi Producing Antimicrobial Compounds From Ketapang Leaves (Terminalia catappa L) Against Resistant Pathogen Bacteria. Macassar. Hasanuddin University.
[3] Ayunda, R. 2015. Isolation, Selection, and Antibacterial Activity Test of Parijoto Leaf Endophytic Mold (Medinilla speciosa Blume) against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Shigella dysenteriae. (Essay). Faculty of Medicine and Health Sciences. Pharmacy Study Program. Jakarta
[4] Ajizah A. Sensitivity of Salmonella Typhimurium to Psidium Guajava L. Bioscientie Leaf Extract. 2004; 1(1): 31-8.
[5] Bhore SJ, Sathisha G. Screening of endophytic colonizing bacteria for cytokinin-like compounds: crude cell-free broth of endophytic colonizing bacteria is unsuitable in cucumber cotyledon bioassay. World J. Agric. science. 2010;6(4):345-52.
[6] Carrol, GC, 1988. Fugal Endophytes In Stems And Leaves From Latent Pathogens To Mutualistic Symbiosis. Ecology, 69:2-9.
[7] Chambers, Henry, F. 2004. The Changing Epidemiology of Staphylococcus aureus. In: CDC Past Issue, vol 7 (2)
[8] Chambers, Henry, F. 2004. The Changing Epidemiology of Staphylococcus aureus. In: CDC Past Issue, vol 7 (2)
[9] Davis and Stout. 1971. Disc Plate Method Of Microbiological Antibiotics. Essay Journal Of Microbiology.22(4): 659-665
[10] Ganiswara, S, 2007, Autonomous Medicine in Pharmacology and Therapy, 5th ed., Department of Pharmacology and Therapeutics, Faculty of Medicine, University of North Sumatra.
[11] Hapsari, Endah, 2015, Anti-Bacterial Test of Meniran Herb Extract (Phyllanthus Niruri) Against the Bacterial Growth of Bacillus cereus and Escherichia Coli, Thesis, Biology Education, Sanata Dharma University : Yokyakarta
[12] Hendri, HZ 2014, Selection of Rational Antibiotics, Medicinus: Vol.27, No.3;40-5. Hendri, HZ2014, Rational Selection of Antibiotics, Medicinus : Vol. 27, No.3;40-5
[13] Jawetz, Melnick, Adelberg. 2013. Medical Microbiology 26th Edition. Jakarta: EGC
[14] Be strong, Arifah. 2013. Inhibitory Test of Chinese Ketepng Leaf Extract (Cassia Alata L) Against the Growth of Pseudomonas Aeruginosa and Streptococcus Muntas Bacteria. Purwokerto ; Faculty of Teacher Training and Education, University of Muhamadiyah Purwokerto.
[15] Maheasy, R., Abdullah, N., Pratiwi, A., Hidayah, M., Ismail, R., 2018. Test the Effectiveness of Ethanol Extract on Chinese Ketepeng Leaves (Cassia alata L.) Against Microbes that Cause Thrush (Aphtosa Stomatitis). Makassar; Alauddin State Islamic University.
[16] Mandell LA, Wundering RG, Anzueto A, Bartlett JG, Campbell GD, Dean NC, DowellSF, File TM Jr. Musher DM, Niederman MS, Torres A, Whitney CG, Infectious Diseases Society of America, American Thoracic Society Clin Infect Dis. 2010 Mar 1; 44 suppl 2:S27-72
[17] Nelson, JM, TM, and Angulo, FJ, 2007, Fluoroquinolone-resistant Campylobacter species and the withdrawal of fluoroquinolones from use in poultry: A public health success story. Clin Infect Dis 44 (7): 977-980.
[18] Nugroho AW, translator. Brooks GF, Carroll KC, Butel JS, Morse SA, Mietzner TA. Jawetz, Melnick, & Adelberg Medical Microbiology. Ed. 25. Jakarta: EGC Medical Book Publisher; 2013.
[19] Pratiwi, Sylvia T. 2008. Pharmaceutical Microbiology. Jakarta: Erlangga Publisher.
[20] Daughter, Delvi Rucitaini. 2016. Comparison of the Effectiveness of Terbinafine With Chinese Ketepeng Leaf Extract (Cassia alata L) Against Fungal Growth
[21] Pratita, Maria Yuli, and Surya Rosa Putra. "Isolation Characterization and Antimicrobial Test of Endophytic Bacteria from Isolation," Scientific Journal of Biology Education, Educational Biology Volume 4, Number 1, June 2012: Pages 7-10.
[22] Radji, Maksum, 2011, Textbook of Microbiology, Student Guide for Pharmacy and Medicine, Jakarta: EGC, pp.10-12,179-199
[23] Safrida, Yuni. 2012. Isolation and Characteristics of Potentially Probiotic Bacteria in Mackerel (Rastrelliger sp) Journal. Syiah Kuala University: Aceh.
[24] Simarmata, R, S. Lekatompessy, and Sukimah, H. 2007. Isolation of Endophytic Microbes from Medicinal Plants Connecting Life (Gymuraprocumbens) and Analysis of Potential as Antimicrobials. Berk Penel Hayati. Vol. 3 (13): 85-90.
[25] Sulistyawati, D., and S. Mulyati. 2009.Test activity of cashew leaf infusion (Anacardiumoccidentale L.) against Candida albicans. Biomedicine.; 2(1): 47-51
[26] Suarni, Jones P., Rosmini. 2017. Inhibitory Test of Chinese Ketepeng Leaf Extract (Cassia Alata L) against Pathogenic Fungus Phytophora Palmivora causing Cocoa Fruit Rot Disease (Theobromae Cacao L.) Faculty of Agriculture, University of Tadulako. Palu
[27] Utami, ER 2011. Antibiotics, Resistance, and Therapeutic Rationality.
[28] Utami, ER 2012. Antibiotics, Resistance, and Therapeutic Rationality. Scientist 1(1):124-138 Scientist. Vol. 1. No. April 1-September.
[29] Waluyo, L. 2007. General Microbiology. University of Muhammadiyah Malang
[30] WHO. World Health Statistics: World Health Statistics 2015. Geneva; 2015; p. 55-86.