EXT COMBINATION ACTIVITIES WHITE WOOD SHELF (Melaleucaleucadendra) AND HONEY AS NON-SPECIFIC IMUNOMODULATOR IN MALE WHITE RATS (Rattusnorvegicus)
Kata Kunci:
Bahasa Indonesia
Abstrak
Mita Kurniawati, 2120192043. 2021. The Combination of Eucalyptus Extract (Melaleuca leucadendra) and Honey as Non-Specific Immunomodulators in Male White Rats (Rattus norvegicus). Undergraduate Thesis, advisor I: Dr. Laksmyn Kadir, M.Kes., and advisor II: Apt. Moh. Taufik Ismullah, M.Farm. Bachelor of Pharmacy, Faculty of Science, Technology, and Health Sciences, Universitas Bina Mandiri Gorontalo.
This study aims to find out the combination of eucalyptus leaf extract (Melaleuca leucadendra) and honey that can provide non-specific Immunomodulators activity in male white rats (Rattus norvegicus).
The method in this study used quasi-experiments with a pretest-posttest approach that used test groups and comparison groups. The groups were: C1 (negative control), C2 (positive control), and C3 (test group).
The results showed that three test groups of a combination of eucalyptus leaf extract (Melaleuca leucadendra) and honey can increase the levels of leukocytes in the blood that serve as Immunomodulators. Analysis of paired sample t-test statistical data where the significance value of P ≤ 0.05 showed that there was activity between a combination of eucalyptus (Melaleuca leucadendra) and honey to increase the levels of leukocytes in the blood that function as immunomodulators.
Keywords: Immunomodulators, Honey, Leukocytes
Referensi
[1] Aden, R. 2010. Regarding Diseases and Other Disorders in Children. Creator's Hangar Cycle. Yogyakarta.
[2] Ahmed, S.; Solomon, SA; Baig, AA; Ibrahim, M.; Liaqat, S.; Fatima, S.; Jabeen, S.; Shamim, N.; Othman, NH 2018. Honey as a Potential Natural Antioxidant Medicine: An Insight into Its Molecular Mechanisms of Action. Oxide. Med. Cells. Longev., 8367846.
[3] Adinugraha et al. 2014. Development of breadfruit (Artocarpus altilis) cultivation techniques for food security. IPB Press
[4] Akbar, Sa'dun. 2013. Learning Device Instruments. Bandung: Rosdakarya.
[5] Al-Sayed, E., El-Lakkany, NM, Seif el-Din, SH, Sabra, A.-NA, & Hammam, OA 2014. Hepatoprotective and antioxidant activity of Melaleuca styphelioideson carbon tetrachloride-induced hepatotoxicity in mice. Pharmaceutical Biology, 52(12), 1581–1590. doi:10.3109/13880209.2014.90839
[6] Angela ES, Davis WL. 2010. Immune-modifying and antimicrobial effects of eucalyptus oil and simple inhalation devices. Alternative Medicine Review. 15(1):33-47
[7] Erywiyatno L, Djoko, Krihariani D. Effect of honey on the growth of Streptococcus pyogenes bacteria. Health Science Analysis. 2012;1(1): 30-7
[8] Al-Hatamleh, MAI; Boers, JC; Wilson, KL; Plebanski, M.; Mohamud, R.; Mustafa, MZ 2020. Antioxidant-Based Medicinal Properties of Stingless Bee Products: Recent Progress and Future Directions. Biomolecules, 10, 923.
[9] Baratawidjaja, et al 2009. Basic Immunology, Eighth Edition. Indonesian Faculty of Medicine Publishing Center. Jakarta
[10] Cianciosi, D.; Forbes-Hernandez, TY; Afrin, S.; Gasparrini, M.; Reboredo-Rodriguez, P.; Manna, PP; Zhang, J.; Bravo Lamas, L.; Martinez Flores, S.; Agudo Toyos, P.; et al. 2018. Phenolic Compounds in Honey and Their Associated Health Benefits: A Review. Molecules 23, 2322
[11] Douglas JW, Wardrop KJ. 2010. Schalm's Veterinary Hematology. 6th ed. Wiley-Blackwell. p852-887.
[12] European Medicines Agency (EMA). 2014. Assessment report on Eucalytus globulus labil., Eucalyptus polybractea RT Baker and/or Eucalyptus smithii RT Baker, aetheroleum. United Kingdom, European Medicines Agency. London
[13] Febriansyah. (2009). Hesperidin as a Preventive Resistance Agent in MCF-7 Breast Cancer Cells Line Resistance to Doxorubicin. Asian Pac J Trop. biomed. 4(3): 228-233.
[14] Gairola, A., Tiwari, P, and Tiwari, JK 2013. Physico-chemical Properties of Apis cerana-indica F. Honey from Uttarkashi District of Uttarakhand, India. Journal of Global Biosci 20-25.
[15] Gridley DS, Pecaut MJ. 2016. Changes in the distribution and function of leukocytes after whole-body iron ion irradiation. J Radiat Res. 57(5):477– 491
[16] Gu, B.; You, J.; Li, Y.; Duan, C.; Fang, M. 2010. Enteric-coated garlic supplement markedly enhanced normal mice immunocompetence. euros. Food Res. Technol., 230, 627−634
[17] Hariana, A. 2009. Medicinal Plants and Their Benefits Series 3. First Edition. Self-Help Spreader. Jakarta
[18] Hudri, FA, 2014. Testing the Effectiveness of Multiflora Honey in Inhibiting the Growth of Salmonella typhi Bacteria. Scientific papers. Faculty of Medicine and Health Sciences Syarif Hidayatullah State Islamic University Jakarta. Jakarta
[19] Istiqomah.2013. Comparison of Maceration and Soxhletation Extraction Methods on Piperine Levels of Javanese Chili (Piperis Retrofracti Fructus). Essay. UIN Jakarta
[20] Irvan, Putra B. 2015. Extraction of 1,8-Cineole from Eucalyptus Urophylla Leaf Oil by Soxhletation Method. USU Journal of Chemical Engineering, Vol. 4, No. 3
[21] Istiqomah. 2013. Comparison of Maceration and Soxhletation Extraction Methods on Piperine Levels in Javanese Chili (Piperis Retrofracti Fructus). Essay. UIN Jakarta
[22] Jin, J. 2012. Methyl n-nonylketone as an active principle of Herba Houtltuyniae and its mechanism. Zhongguo Linchuang Yaoxue Zazhi, 21(1), 23−26.
[23] Kartikawati, NK, et al. 2014. Cultivation and Development Prospects of Eucalyptus (Melaleuca cajuputi). IPB Press. Bogor
[24] Kana, M., 2017. The Effect of Honey on T Lymphocyte Levels in Breast Cancer Patients With Chemotherapy At Dr. Hospital. Wahidin Sudirohusodo Makassar. Thesis. Faculty of Medicine, Graduate Program, Hasanuddin University Makassar. Makassar
[25] Khan, SU; Anjum, SI; Rahman, K.; Ansari, MJ; Khan, WU; Kamal, S.; Khattak, B.; Muhammad, A.; Khan, HU 2018. Honey: Single food stuff comprises many drugs. Saudi J. Biol. Sci., 25, 320–325.
[26] Li, Y, J., Zhong, ZX., Lin, X., Liu, BM., 2013. Study on pharmacological action of aqueous extract from Melaleuca ahemifolia. Guit. J. Tradit. Chin. Med. Pharm. 5, 041
[27] Linda K, Widyarti S. Effect of ethanol extract of soursop leaf (Annona muricata Linn.) on increasing the number of CD4+ and CD8+ T cells in the thymus of mice (Mus musculus). Research report. Brawijaya University. 2011; 2426.
[28] Miguel, MG; Antunes, MD; Faleiro, ML 2017. Honey as a Complementary Medicine. Integra. Med. Insights., 12.
[29] Majtan, J., Bohova, J., Garcia-Villalba, R., Tomas-Barberan, FA, Madakova, Z., Majtan, T., … Klaudiny, J. (2013). Fir honey dew honey flavonoids inhibit TNF-α induced MMP-9 expression in human keratinocytes: A new action of honey in wound healing. Archives of Dermatological Research, 305(7), 619–627.
[2] Ahmed, S.; Solomon, SA; Baig, AA; Ibrahim, M.; Liaqat, S.; Fatima, S.; Jabeen, S.; Shamim, N.; Othman, NH 2018. Honey as a Potential Natural Antioxidant Medicine: An Insight into Its Molecular Mechanisms of Action. Oxide. Med. Cells. Longev., 8367846.
[3] Adinugraha et al. 2014. Development of breadfruit (Artocarpus altilis) cultivation techniques for food security. IPB Press
[4] Akbar, Sa'dun. 2013. Learning Device Instruments. Bandung: Rosdakarya.
[5] Al-Sayed, E., El-Lakkany, NM, Seif el-Din, SH, Sabra, A.-NA, & Hammam, OA 2014. Hepatoprotective and antioxidant activity of Melaleuca styphelioideson carbon tetrachloride-induced hepatotoxicity in mice. Pharmaceutical Biology, 52(12), 1581–1590. doi:10.3109/13880209.2014.90839
[6] Angela ES, Davis WL. 2010. Immune-modifying and antimicrobial effects of eucalyptus oil and simple inhalation devices. Alternative Medicine Review. 15(1):33-47
[7] Erywiyatno L, Djoko, Krihariani D. Effect of honey on the growth of Streptococcus pyogenes bacteria. Health Science Analysis. 2012;1(1): 30-7
[8] Al-Hatamleh, MAI; Boers, JC; Wilson, KL; Plebanski, M.; Mohamud, R.; Mustafa, MZ 2020. Antioxidant-Based Medicinal Properties of Stingless Bee Products: Recent Progress and Future Directions. Biomolecules, 10, 923.
[9] Baratawidjaja, et al 2009. Basic Immunology, Eighth Edition. Indonesian Faculty of Medicine Publishing Center. Jakarta
[10] Cianciosi, D.; Forbes-Hernandez, TY; Afrin, S.; Gasparrini, M.; Reboredo-Rodriguez, P.; Manna, PP; Zhang, J.; Bravo Lamas, L.; Martinez Flores, S.; Agudo Toyos, P.; et al. 2018. Phenolic Compounds in Honey and Their Associated Health Benefits: A Review. Molecules 23, 2322
[11] Douglas JW, Wardrop KJ. 2010. Schalm's Veterinary Hematology. 6th ed. Wiley-Blackwell. p852-887.
[12] European Medicines Agency (EMA). 2014. Assessment report on Eucalytus globulus labil., Eucalyptus polybractea RT Baker and/or Eucalyptus smithii RT Baker, aetheroleum. United Kingdom, European Medicines Agency. London
[13] Febriansyah. (2009). Hesperidin as a Preventive Resistance Agent in MCF-7 Breast Cancer Cells Line Resistance to Doxorubicin. Asian Pac J Trop. biomed. 4(3): 228-233.
[14] Gairola, A., Tiwari, P, and Tiwari, JK 2013. Physico-chemical Properties of Apis cerana-indica F. Honey from Uttarkashi District of Uttarakhand, India. Journal of Global Biosci 20-25.
[15] Gridley DS, Pecaut MJ. 2016. Changes in the distribution and function of leukocytes after whole-body iron ion irradiation. J Radiat Res. 57(5):477– 491
[16] Gu, B.; You, J.; Li, Y.; Duan, C.; Fang, M. 2010. Enteric-coated garlic supplement markedly enhanced normal mice immunocompetence. euros. Food Res. Technol., 230, 627−634
[17] Hariana, A. 2009. Medicinal Plants and Their Benefits Series 3. First Edition. Self-Help Spreader. Jakarta
[18] Hudri, FA, 2014. Testing the Effectiveness of Multiflora Honey in Inhibiting the Growth of Salmonella typhi Bacteria. Scientific papers. Faculty of Medicine and Health Sciences Syarif Hidayatullah State Islamic University Jakarta. Jakarta
[19] Istiqomah.2013. Comparison of Maceration and Soxhletation Extraction Methods on Piperine Levels of Javanese Chili (Piperis Retrofracti Fructus). Essay. UIN Jakarta
[20] Irvan, Putra B. 2015. Extraction of 1,8-Cineole from Eucalyptus Urophylla Leaf Oil by Soxhletation Method. USU Journal of Chemical Engineering, Vol. 4, No. 3
[21] Istiqomah. 2013. Comparison of Maceration and Soxhletation Extraction Methods on Piperine Levels in Javanese Chili (Piperis Retrofracti Fructus). Essay. UIN Jakarta
[22] Jin, J. 2012. Methyl n-nonylketone as an active principle of Herba Houtltuyniae and its mechanism. Zhongguo Linchuang Yaoxue Zazhi, 21(1), 23−26.
[23] Kartikawati, NK, et al. 2014. Cultivation and Development Prospects of Eucalyptus (Melaleuca cajuputi). IPB Press. Bogor
[24] Kana, M., 2017. The Effect of Honey on T Lymphocyte Levels in Breast Cancer Patients With Chemotherapy At Dr. Hospital. Wahidin Sudirohusodo Makassar. Thesis. Faculty of Medicine, Graduate Program, Hasanuddin University Makassar. Makassar
[25] Khan, SU; Anjum, SI; Rahman, K.; Ansari, MJ; Khan, WU; Kamal, S.; Khattak, B.; Muhammad, A.; Khan, HU 2018. Honey: Single food stuff comprises many drugs. Saudi J. Biol. Sci., 25, 320–325.
[26] Li, Y, J., Zhong, ZX., Lin, X., Liu, BM., 2013. Study on pharmacological action of aqueous extract from Melaleuca ahemifolia. Guit. J. Tradit. Chin. Med. Pharm. 5, 041
[27] Linda K, Widyarti S. Effect of ethanol extract of soursop leaf (Annona muricata Linn.) on increasing the number of CD4+ and CD8+ T cells in the thymus of mice (Mus musculus). Research report. Brawijaya University. 2011; 2426.
[28] Miguel, MG; Antunes, MD; Faleiro, ML 2017. Honey as a Complementary Medicine. Integra. Med. Insights., 12.
[29] Majtan, J., Bohova, J., Garcia-Villalba, R., Tomas-Barberan, FA, Madakova, Z., Majtan, T., … Klaudiny, J. (2013). Fir honey dew honey flavonoids inhibit TNF-α induced MMP-9 expression in human keratinocytes: A new action of honey in wound healing. Archives of Dermatological Research, 305(7), 619–627.
Diterbitkan
2023-10-02
##submission.howToCite##
Mita Kurniawati. (2023). EXT COMBINATION ACTIVITIES WHITE WOOD SHELF (Melaleucaleucadendra) AND HONEY AS NON-SPECIFIC IMUNOMODULATOR IN MALE WHITE RATS (Rattusnorvegicus). Journal of Health, Technology and Science (JHTS), 4(3), 225-239. https://doi.org/10.47918/jhts.v4i3.429
Bagian
Articles
##submission.copyrightStatement##
##submission.license.cc.by4.footer##
