ANTIBACTERIAL TEST OF Basil (Ocimum sanctum) LEAF EXTRACT AND FRACTION ON Escherichia coli BACTERIA GROWTH
Abstrak
Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak dan fraksi daun kemangi dalam menghambat pertumbuhan bakteri Escherichia coli dan untuk mengetahui konsentrasi yang terbaik dalam menghambat pertumbuhan bakteri Escherichia coli.
Metode dalam penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan lima perlakuan dan lima pengulangan berdasarkan masing-masing kelompok perlakuan ekstrak dan fraksi yaitu: konsentrasi 25%, konsentrasi 50% dan konsentrasi 75%.
Hasil penelitian menunjukan bahwa dari masing-masing konsentrasi yang digunakan dari kelompok ekstrak dan fraksi didapatkan bahwa konsentrasi terbesar dalam menghambat bakteri Eschericia coli pada konsentrasi 75% kelompok ekstrak. Analisa data secara statistik menggunakan Metode Kruskal Wallis terdapat perbedaan nilai yang signifikan antara ekstrak dan fraksi sehingga dilanjutkan dengan menggunakan uji Post Huc Duncan.
Kata Kunci : Kemangi, Ekstrak, Fraksi, Escherichia coli
Referensi
1. There is an effect of extracts and fractions from basil leaves (Ocimum sanctum) on the growth of Escherichia coli bacteria, this is evidenced by the presence of an inhibitory zone formed around the paper disc.
2. The best concentration in inhibiting the growth of Escherichia coli bacteria was 75% concentration in the fraction treatment.
BIBLIOGRAPHY
[1] Agusta, A. 2000. Indonesian tropical plant essential oils, 5,9,18-19,23,89. ITB : Bandung
[2] Atikah, N. 2013. Antimicrobial Activity Test of Basil Herb Extract (Ocimum americanum L) against Staphylococcus aureus and Candida albicans. Essay. Jakarta: UIN Syarif Hidayatullah Jakarta.
[3] Atika Threenesia. 2019. Comparison of Effects of Ethanol Extract of Basil Leaves (Ocimum sanctum L.) on In Vitro Growth Inhibitory Power of Staphylococcus aureus and Salmonella typhi. Faculty of Medicine, University of Lampung : Bandar Lampung.
[4] Babu, VS & DSKSarma. 2011. Pharmacognostic and Phyochemical Studies of Ocimum americanum. Journal of Chemical and Pharmaceutical Research, 3 (3) : 337-347
[5] Bettleheim KA (2000). Role of non O157 VTEC. J. Appl. sym. Microbiol. suppl.
[6] Cindy, White. 2005. The Impact of a Health Campaign on Hand Hygiene and Upper Respiratory Illness Among College Students Living in Residence Halls. Journal of American College Health, 53(4): 175-181
[7] Davis, WW and Stout, TR 1971. Disc Plate Method of Microbiological Antibiotic Assay. Applied Microbiology. 22(4): 659-665.
[8] Directorate General of POM. 1986. Galenic Preparations. Indonesian Ministry of Health: Jakarta
[9] Diyah, beautiful. 2016. Potential Effects of Basil Leaves (Ocimum basilicum L.) as Hand Sanitizer Utilization. Faculty of Medicine, University of Lampung : Bandar Lampung
[10] Indonesian Ministry of Health, Directorate General of PPM & PL 2005, Decree of the Minister of Health of the Republic of Indonesia no 1216/MENKES/SK/XI/2001 concerning Guidelines for the Eradication of Diarrhea, 4th edition.
[11] Dhulgande, G., ARBirari & DADhale. 2010. Preliminary Screening of Antibacterial and Phytochemical Studies of Ocimum americanum Linn. Journal of Ecobiotechnology, 2 (8) : 11-13
[12] Dufour, AP 1984. Health Effects Criteria for Fresh Recreational Waters. Cincinnati, Ohio, US Environmental Protection Agency, EPA-600/1-84-004, 33p.
[13] Dwidjoseputro. 2005.Microbiology Fundamentals. bridge. Jakarta
[14] Escherich, T. 1885. Die Darmbakterien des Neugeborenen und Sauglings. Fortschr. Med. 3: 515-522; 547-554.
[15] Festy, Adriyanaastri. 2012.Etiology and clinical features of acute diarrhea at Dr Kariadi Hospital, Semarang. University School of Medicine
Diponegoro : Semarang
[16] Fitriani T. 2014. The effectiveness of basil leaf extract (Ocimim Basilicum L.) on reducing levels of volatile sulfur compounds (vscs) [thesis]. Makassar: Hasanudin University
[17] Ganiswarna, SG, 2007, Pharmacology and Therapy 5th Edition, Department of Pharmacology and Therapeutics, Faculty of Medicine UI, Jakarta
[18] Gulo, W. 2002. Research Methodology. Grasindo : Jakarta
[19] Gunardi, D. 2010. Separation of Basil (Ocimum basilicum Linn) Essential Oil by Thin Layer Chromatography and Its Activity against Malassezia Furur. Young Medika Media; 4:63-68
[20] Handayani, PA & M. Safaatul. 2010. Extraction of Kaffir lime leaf oil (Citrus hystrix DC) with ethanol and N-hexane as solvents. Journal of Technical Competence. 2 (1).
[21] Harborne, JB, 1987, Phytochemical Methods, A Guide to Modern Ways of Analyzing Plants, translated by Dr. Kosasih Patmawinata and Dr. Iwang Soediro, ITB. Bandung.
[22] Hasnaeni, 2014. Plant Morphology. Pharmacy Biology Laboratory, Makassar National Pharmacy Academy. Makassar.
[23] Kicel A, A Kurowska and D Kalemba. 2005. Composition of the essential oils ofOcimum sanctum grow in poland during vegetation. J. Essentian Oil Res. 17, 217-219.
[24] Khalil, A., 2013, Antimicrobial Activity of Ethanolic Extracts of Ocimum basilicum Leaf From Saudi Arabia, Biotechnology, Asian Network for Scientific Information, ISSN 1682 – 296X /DOI 10.3293 / bitotech 2013.
[25] Kurniasih. 2014. Powerful Benefits of Basil. Yogyakarta: New Press Library.
[26] Kusuma, W. 2010. Effect of basil leaf extract (Ocimum sanctum L.) on hepatocyte damage in mice due to palm oil with repeated heating. Essay. Faculty of Medicine, Sebelas Maret University: Surakarta.
[27] Mandal Bk, EGL Wilkins, EM Dunbar. And RT Mayon-White. Lecture notes on infectious diseases. Erlangga. 2008
[28] Mardia. 2017. Test of Staphylococcus aureus Resistance to Amoxcillin, Tetracycline and Propolis Antibiotics. Muhammadiyah Academy of Health Analyst. Makassar
[29] Margono. 2004. Educational research methodology. Jakarta : Rineka Cipta
[30] Maryati. Antibacterial activity test of basil leaf essential oil (Ocimum basilicum l.) against staphylococcus aureus and escherechia coli. 2007; 8(1)30-38.
[31] Maulida, D. and Zulkarnaen, N. 2010. Extraction of antioxidants (lycopene) from tomatoes using a mixed solvent of n-hexane, acetone and ethanol, Thesis. Faculty of Engineering. Diponegoro University
[32] Monem MA., Mohamed EA., Awad ET., Ramadan AHM., and Mahmoud HA. (2014). Multiplex PCR as emerging technique for diagnosis of enterotoxigenic E. Coli isolates from pediatric watery diarrhea. Journal of American Science, Vol 10 No (10)
[33] Rachmawaty. 2016.Phenolic Levels and Antioxidant Activity in Five Accessions of Turmeric Plants at Cultivation Sites in Nagrak District. Essay. Faculty of Mathematics and Natural Sciences. IPB Bandung
[34] Rahayu, T., RS Fauzia & Maryati. Antibacterial Activity Test of Basil Leaf Essential Oil (Ocimum basilicum L) against Staphylococcus aureus and Escherichia coli. Journal of Science and Technology Research, 8 (1) : 30 – 38
[35] Robinson, T. 1995. Organic Chemical Content of High Plants. Translated by Kosasih Padmawinta, K. Publisher ITB, Bandung.
[36] Rosenthal, Victor D. 2005. Reduction in nosocomial infection with improved hand hygiene in intensive care units of a tertiary care hospital in Argentina. American Journal of Infection Control, 33(7): 392–397
[37] Schaffner, Donald W. 2007. Management of Risk of Microbial Cross Contamination from Uncooked Frozen Hamburgers by Alcohol Based Hand Sanitizer. Journal of Food Protection, 1: 109-113
[38] Shulman, ST, Bisno, AL, Clegg, HW, Gerber, MA, Kaplan, EL, Lee, G., and Van Beneden, C. 2012. Clinical practice guideline for the diagnosis and management of group a Streptococcal pharyngitis: 2012 update by the infectious diseases society of America. Clinical Infectious Diseases. 55(10): 86–102.
[39] Sugiyono (2015). Combination Research Methods (Mix Methods). Bandung: Alphabeta.
[40] Sunarjati. 2015. Mechanism of Antibiotic Resistance in Bacterial Infections.Microbiology
2. The best concentration in inhibiting the growth of Escherichia coli bacteria was 75% concentration in the fraction treatment.
BIBLIOGRAPHY
[1] Agusta, A. 2000. Indonesian tropical plant essential oils, 5,9,18-19,23,89. ITB : Bandung
[2] Atikah, N. 2013. Antimicrobial Activity Test of Basil Herb Extract (Ocimum americanum L) against Staphylococcus aureus and Candida albicans. Essay. Jakarta: UIN Syarif Hidayatullah Jakarta.
[3] Atika Threenesia. 2019. Comparison of Effects of Ethanol Extract of Basil Leaves (Ocimum sanctum L.) on In Vitro Growth Inhibitory Power of Staphylococcus aureus and Salmonella typhi. Faculty of Medicine, University of Lampung : Bandar Lampung.
[4] Babu, VS & DSKSarma. 2011. Pharmacognostic and Phyochemical Studies of Ocimum americanum. Journal of Chemical and Pharmaceutical Research, 3 (3) : 337-347
[5] Bettleheim KA (2000). Role of non O157 VTEC. J. Appl. sym. Microbiol. suppl.
[6] Cindy, White. 2005. The Impact of a Health Campaign on Hand Hygiene and Upper Respiratory Illness Among College Students Living in Residence Halls. Journal of American College Health, 53(4): 175-181
[7] Davis, WW and Stout, TR 1971. Disc Plate Method of Microbiological Antibiotic Assay. Applied Microbiology. 22(4): 659-665.
[8] Directorate General of POM. 1986. Galenic Preparations. Indonesian Ministry of Health: Jakarta
[9] Diyah, beautiful. 2016. Potential Effects of Basil Leaves (Ocimum basilicum L.) as Hand Sanitizer Utilization. Faculty of Medicine, University of Lampung : Bandar Lampung
[10] Indonesian Ministry of Health, Directorate General of PPM & PL 2005, Decree of the Minister of Health of the Republic of Indonesia no 1216/MENKES/SK/XI/2001 concerning Guidelines for the Eradication of Diarrhea, 4th edition.
[11] Dhulgande, G., ARBirari & DADhale. 2010. Preliminary Screening of Antibacterial and Phytochemical Studies of Ocimum americanum Linn. Journal of Ecobiotechnology, 2 (8) : 11-13
[12] Dufour, AP 1984. Health Effects Criteria for Fresh Recreational Waters. Cincinnati, Ohio, US Environmental Protection Agency, EPA-600/1-84-004, 33p.
[13] Dwidjoseputro. 2005.Microbiology Fundamentals. bridge. Jakarta
[14] Escherich, T. 1885. Die Darmbakterien des Neugeborenen und Sauglings. Fortschr. Med. 3: 515-522; 547-554.
[15] Festy, Adriyanaastri. 2012.Etiology and clinical features of acute diarrhea at Dr Kariadi Hospital, Semarang. University School of Medicine
Diponegoro : Semarang
[16] Fitriani T. 2014. The effectiveness of basil leaf extract (Ocimim Basilicum L.) on reducing levels of volatile sulfur compounds (vscs) [thesis]. Makassar: Hasanudin University
[17] Ganiswarna, SG, 2007, Pharmacology and Therapy 5th Edition, Department of Pharmacology and Therapeutics, Faculty of Medicine UI, Jakarta
[18] Gulo, W. 2002. Research Methodology. Grasindo : Jakarta
[19] Gunardi, D. 2010. Separation of Basil (Ocimum basilicum Linn) Essential Oil by Thin Layer Chromatography and Its Activity against Malassezia Furur. Young Medika Media; 4:63-68
[20] Handayani, PA & M. Safaatul. 2010. Extraction of Kaffir lime leaf oil (Citrus hystrix DC) with ethanol and N-hexane as solvents. Journal of Technical Competence. 2 (1).
[21] Harborne, JB, 1987, Phytochemical Methods, A Guide to Modern Ways of Analyzing Plants, translated by Dr. Kosasih Patmawinata and Dr. Iwang Soediro, ITB. Bandung.
[22] Hasnaeni, 2014. Plant Morphology. Pharmacy Biology Laboratory, Makassar National Pharmacy Academy. Makassar.
[23] Kicel A, A Kurowska and D Kalemba. 2005. Composition of the essential oils ofOcimum sanctum grow in poland during vegetation. J. Essentian Oil Res. 17, 217-219.
[24] Khalil, A., 2013, Antimicrobial Activity of Ethanolic Extracts of Ocimum basilicum Leaf From Saudi Arabia, Biotechnology, Asian Network for Scientific Information, ISSN 1682 – 296X /DOI 10.3293 / bitotech 2013.
[25] Kurniasih. 2014. Powerful Benefits of Basil. Yogyakarta: New Press Library.
[26] Kusuma, W. 2010. Effect of basil leaf extract (Ocimum sanctum L.) on hepatocyte damage in mice due to palm oil with repeated heating. Essay. Faculty of Medicine, Sebelas Maret University: Surakarta.
[27] Mandal Bk, EGL Wilkins, EM Dunbar. And RT Mayon-White. Lecture notes on infectious diseases. Erlangga. 2008
[28] Mardia. 2017. Test of Staphylococcus aureus Resistance to Amoxcillin, Tetracycline and Propolis Antibiotics. Muhammadiyah Academy of Health Analyst. Makassar
[29] Margono. 2004. Educational research methodology. Jakarta : Rineka Cipta
[30] Maryati. Antibacterial activity test of basil leaf essential oil (Ocimum basilicum l.) against staphylococcus aureus and escherechia coli. 2007; 8(1)30-38.
[31] Maulida, D. and Zulkarnaen, N. 2010. Extraction of antioxidants (lycopene) from tomatoes using a mixed solvent of n-hexane, acetone and ethanol, Thesis. Faculty of Engineering. Diponegoro University
[32] Monem MA., Mohamed EA., Awad ET., Ramadan AHM., and Mahmoud HA. (2014). Multiplex PCR as emerging technique for diagnosis of enterotoxigenic E. Coli isolates from pediatric watery diarrhea. Journal of American Science, Vol 10 No (10)
[33] Rachmawaty. 2016.Phenolic Levels and Antioxidant Activity in Five Accessions of Turmeric Plants at Cultivation Sites in Nagrak District. Essay. Faculty of Mathematics and Natural Sciences. IPB Bandung
[34] Rahayu, T., RS Fauzia & Maryati. Antibacterial Activity Test of Basil Leaf Essential Oil (Ocimum basilicum L) against Staphylococcus aureus and Escherichia coli. Journal of Science and Technology Research, 8 (1) : 30 – 38
[35] Robinson, T. 1995. Organic Chemical Content of High Plants. Translated by Kosasih Padmawinta, K. Publisher ITB, Bandung.
[36] Rosenthal, Victor D. 2005. Reduction in nosocomial infection with improved hand hygiene in intensive care units of a tertiary care hospital in Argentina. American Journal of Infection Control, 33(7): 392–397
[37] Schaffner, Donald W. 2007. Management of Risk of Microbial Cross Contamination from Uncooked Frozen Hamburgers by Alcohol Based Hand Sanitizer. Journal of Food Protection, 1: 109-113
[38] Shulman, ST, Bisno, AL, Clegg, HW, Gerber, MA, Kaplan, EL, Lee, G., and Van Beneden, C. 2012. Clinical practice guideline for the diagnosis and management of group a Streptococcal pharyngitis: 2012 update by the infectious diseases society of America. Clinical Infectious Diseases. 55(10): 86–102.
[39] Sugiyono (2015). Combination Research Methods (Mix Methods). Bandung: Alphabeta.
[40] Sunarjati. 2015. Mechanism of Antibiotic Resistance in Bacterial Infections.Microbiology
Diterbitkan
2023-10-02
##submission.howToCite##
BecerangS. A. (2023). ANTIBACTERIAL TEST OF Basil (Ocimum sanctum) LEAF EXTRACT AND FRACTION ON Escherichia coli BACTERIA GROWTH. Journal of Health, Technology and Science (JHTS), 4(3), 212-224. https://doi.org/10.47918/jhts.v4i3.431
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